Biological and immunological characterization of corticotropin-releasing activity in the bovine adrenal medulla.


Corticotropin-releasing factor (CRF)-like activity in bovine adrenal medulla extracts were characterized by measurement of adrenocorticotropin (ACTH) release from rat anterior pituitary cells in vitro, and by a sensitive heterologous radioimmunoassay (RIA) for bovine hypothalamic CRF. Bovine adrenal medulla was boiled in 2 M acetic acid, homogenized, and… (More)