Biological and clinical relevance of transcriptionally active human papillomavirus (HPV) infection in oropharynx squamous cell carcinoma

  title={Biological and clinical relevance of transcriptionally active human papillomavirus (HPV) infection in oropharynx squamous cell carcinoma},
  author={Alain Jung and Jenny Briolat and R{\'e}gine Millon and Aur{\'e}lien de Reyni{\`e}s and David S Rickman and Emilie Thomas and Joseph Abecassis and Christine Clavel and Bohdan Wasylyk},
  journal={International Journal of Cancer},
Human papillomaviruses (HPV) are associated with a subset of head and neck squamous cell carcinoma (HNSCC), particularly HPV16. This study analyzed the presence and genotype of high risk HPVs, viral DNA load and transcription of the E6/E7 mRNAs, in 231 consecutive HNSCC. Twelve out of 30 HPV16 DNA‐positive tumors displayed high E6/E7 mRNAs levels and were localized in the oropharyngeal region. While HPV‐free and non‐transcriptionally active HPV‐related patients showed similar 5‐years survival… 

The role of HPV RNA transcription, immune response‐related gene expression and disruptive TP53 mutations in diagnostic and prognostic profiling of head and neck cancer

It is found that tumors with transcriptionally inactive HPV16 (DNA+ RNA‐) are similar to HPV‐negative (DNA‐) tumors regarding gene expression and frequency of TP53 mutations and that an immune response‐related gene expression cluster is associated with lymph node metastasis, independent of HPV16 status.

A comprehensive evaluation of human papillomavirus positive status and p16INK4a overexpression as a prognostic biomarker in head and neck squamous cell carcinoma

Multivariate analysis revealed that T1-3 stage and the combination of HPV DNA positivity and p16INK4a overexpression predicted significantly better recurrence-free survival and offers a useful and reliable method for detecting and determining the prognosis of HPV-related HNSCC.

Human Papillomavirus Infection in Head and Neck Squamous Cell Carcinomas: Transcriptional Triggers and Changed Disease Patterns

Current evidence on level of a subset of host transcription factors that influence viral genome, directly or indirectly, in head and neck squamous cell carcinoma is reviewed and evaluated.

Sensitivity and specificity of antibodies against HPV16 E6 and other early proteins for the detection of HPV16‐driven oropharyngeal squamous cell carcinoma

HPV‐driven Oropharyngeal squamous cell carcinoma patients from Germany and Italy with fresh‐frozen tumor tissues and sera collected before treatment were included in this study, and HPV16 E6 seropositivity appears to be a highly reliable diagnostic marker for HPV16‐driven OPSCC with very high sensitivity and specificity.

Advances in Tumor Virology Role of HPV in Head and Neck Cancer

The experimental and epidemiological data provide robust evidence for a causal role in OPSCC, but the evidence for association of HPV with other HNSCC such as oral cavity or larynx is weak.

Low prevalence of HPV-driven head and neck squamous cell carcinoma in North-East Italy

High levels of HPV16-L1 antibody but not HPV16 DNA load or integration predict oropharyngeal patient outcome: The Papillophar study

It is revealed that HPV16 load and integration are not relevant prognosis biomarkers in OPC patients, and high anti-HPV16 L1 antibody titers were significantly associated with a better OS and PFS.

Identification of High-Risk Human Papillomavirus DNA, p16, and E6/E7 Oncoproteins in Laryngeal and Hypopharyngeal Squamous Cell Carcinomas

A high prevalence of HPV16 as a primary HR-HPV type in LSCC and HPSCC is suggested and the lack of HPV E6/E7 oncoproteins in some tumor samples may suggest either the absence of viral integration or the presence of other mechanisms of tumorigenesis.

Virology and molecular pathogenesis of HPV (human papillomavirus)-associated oropharyngeal squamous cell carcinoma.

The present review summarizes the aetiology of HPV-positive (HPV+) OPSCC and provides a detailed overview of HPV virology and molecular pathogenesis relevant to infection of oropharyngeal tissues.



Gene Expression Differences Associated with Human Papillomavirus Status in Head and Neck Squamous Cell Carcinoma

Gene expression profiles of HPV+ and HPV− tumors were compared with further exploration of the biological effect of HPV in HNSCC to reveal the unique pathways in HPV+ tumors that may explain the different natural history and biological properties of these tumors.

Molecular classification identifies a subset of human papillomavirus--associated oropharyngeal cancers with favorable prognosis.

  • P. WeinbergerZiwei Yu A. Psyrri
  • Medicine, Biology
    Journal of clinical oncology : official journal of the American Society of Clinical Oncology
  • 2006
A novel classification scheme that may have value for patient stratification for clinical trials testing HPV-targeted therapies is defined, namely HPV+/p16 high (class III) in oropharyngeal squamous cell carcinoma.

A subset of head and neck squamous cell carcinomas exhibits integration of HPV 16/18 DNA and overexpression of p16INK4A and p53 in the absence of mutations in p53 exons 5–8

Evaluation of clinical data revealed a significant inverse relation between tobacco use with or without alcohol consumption, and HPV positivity of the tumors, and FISH is considered to be less sensitive than PCR‐based methods for HPV detection.

Detection of human papillomavirus DNA and oncoprotein overexpression are associated with distinct morphological patterns of tonsillar squamous cell carcinoma.

The morphology of the HPV-positive tumors suggests that HPV may have a predilection for a population of nonkeratinizing squamous cells or that the virally transformed cells inhibit keratinization of the tumor cells.

Genome-wide DNA copy number alterations in head and neck squamous cell carcinomas with or without oncogene-expressing human papillomavirus

Detailed genome analysis of head and neck squamous cell carcinomas with and without HPV16 involvement shows that HNSCC arising by environmental carcinogens are characterized by genetic alterations that differ from those observed in HPV16-induced H NSCC, and most likely occur early in carcinogenesis.

HPV in oral squamous cell carcinoma vs head and neck squamous cell carcinoma biopsies: a meta-analysis (1988-2007).

Findings support the assumption that a correct distinction of HNSCC by site, together with the use of more sensitive HPV DNA detection methods, should be considered as essential prerogatives in designing future investigations into viral prevalence in head and neck tumors.

Involvement of intact HPV16 E6/E7 gene expression in head and neck cancers with unaltered p53 status and perturbed pRb cell cycle control

The data demonstrate that HPV16 DNA-positivity in head and neck cancers is not indicative of a causal role, and the E6 gene is found to be disrupted in most p53-mutated tumours without E6/E7 expression.

Evidence for a causal association between human papillomavirus and a subset of head and neck cancers.

It is suggested that HPV-positive oropharyngeal cancers comprise a distinct molecular, clinical, and pathologic disease entity that is likely causally associated with HPV infection and that has a markedly improved prognosis.