Biological and biochemical properties of human rasH genes mutated at codon 61.

@article{Der1986BiologicalAB,
  title={Biological and biochemical properties of human rasH genes mutated at codon 61.},
  author={Channing J Der and Toren Finkel and Geoffrey M. Cooper},
  journal={Cell},
  year={1986},
  volume={44 1},
  pages={167-76}
}
Using site-directed mutagenesis, we have introduced mutations encoding 17 different amino acids at codon 61 of the human rasH gene. Fifteen of these substitutions increased rasH transforming activity. The remaining two mutants, encoding proline and glutamic acid, displayed transforming activities similar to the normal gene. Overall, these mutants vary over 1000-fold in transforming potency. Increased levels of p21 expression were required for transformation by weakly transforming mutants. The… CONTINUE READING

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