Biological Macromolecules : UV-visible Spectrophotometry

  title={Biological Macromolecules : UV-visible Spectrophotometry},
  author={F Schmid},
Spectroscopy is a technique that measures the interaction of molecules with electromagnetic radiation. Light in the near-ultraviolet (UV) and visible (vis) range of the electromagnetic spectrum has an energy of about 150– 400 kJmol . The energy of the light is used to promote electrons from the ground state to an excited state. A spectrum is obtained when the absorption of light is measured as a function of its frequency or wavelength. Molecules with electrons in delocalized aromatic systems… CONTINUE READING


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Optical spectroscopy to characterize protein conformation and conformational changes

  • TE Creighton
  • Protein Structure : A Practical Approach
  • 1997

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