Biolistic transformation of highly regenerative sugar beet (Beta vulgaris L.) leaves

@article{IvicHaymes2004BiolisticTO,
  title={Biolistic transformation of highly regenerative sugar beet (Beta vulgaris L.) leaves},
  author={Snezana D. Ivic-Haymes and Ann C. Smigocki},
  journal={Plant Cell Reports},
  year={2004},
  volume={23},
  pages={699-704}
}
Leaves of greenhouse-grown sugar beet (Beta vulgaris L.) plants that were first screened for high regeneration potential were transformed via particle bombardment with the uidA gene fused to the osmotin or proteinase inhibitor II gene promoter. Stably transformed calli were recovered as early as 7 weeks after bombardment and GUS-positive shoots regenerated 3 months after bombardment. The efficiency of transformation ranged from 0.9% to 3.7%, and stable integration of the uidA gene into the… CONTINUE READING
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