Biolayer interferometry of lipid nanodisc-reconstituted yeast vacuolar H+ -ATPase.

Abstract

Vacuolar H+ -ATPase (V-ATPase) is a large, multisubunit membrane protein complex responsible for the acidification of subcellular compartments and the extracellular space. V-ATPase activity is regulated by reversible disassembly, resulting in cytosolic V1 -ATPase and membrane-integral V0 proton channel sectors. Reversible disassembly is accompanied by… (More)
DOI: 10.1002/pro.3143

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