Bioinformatics prediction of miRNAs in the Prunus persica genome with validation of their precise sequences by miR-RACE.

Abstract

We predicted 262 potential MicroRNAs (miRNAs) belonging to 70 miRNA families from the peach (Prunus persica) genome and two specific 5' and 3' miRNA rapid amplification of cDNA ends (miR-RACE) PCR reactions and sequence-directed cloning were employed to accurately validate 61 unique P. persica miRNAs (Ppe-miRNAs) sequences belonging to 61 families… (More)
DOI: 10.1016/j.jplph.2012.08.021

Topics

3 Figures and Tables