Bioinformatics prediction of miRNAs in the Prunus persica genome with validation of their precise sequences by miR-RACE.


We predicted 262 potential MicroRNAs (miRNAs) belonging to 70 miRNA families from the peach (Prunus persica) genome and two specific 5' and 3' miRNA rapid amplification of cDNA ends (miR-RACE) PCR reactions and sequence-directed cloning were employed to accurately validate 61 unique P. persica miRNAs (Ppe-miRNAs) sequences belonging to 61 families… (More)
DOI: 10.1016/j.jplph.2012.08.021


3 Figures and Tables