Bioguided isolation of pentacyclic triterpenes from the leaves of Alstonia scholaris (Linn.) R. Br. growing in Egypt

  title={Bioguided isolation of pentacyclic triterpenes from the leaves of Alstonia scholaris (Linn.) R. Br. growing in Egypt},
  author={Hesham I. El-askary and Mahmoud M. El-Olemy and Maha Mahmoud Salama and Amany Ameen Sleem and Mahetab H. Amer},
  journal={Natural Product Research},
  pages={1755 - 1758}
Ethanolic and aqueous extracts of the leaves and flowers of Alstonia scholaris were evaluated for their antioxidant activity by investigating their effect on blood glutathione levels in alloxan-induced diabetic rats. The ethanolic extract of the leaves was the most active; therefore, its cytotoxicity against HepG2 cells was also tested. Promising GI50 values of 1.96, 4.34 and 4.65 µg mL–1 were observed for the extract, its chloroform and ethyl acetate fractions, respectively. The chloroform… Expand
Antibacterial and Synergistic Activity of Pentacyclic Triterpenoids Isolated from Alstonia scholaris
Findings reflect that pentacyclic triterpenoids are the antibacterial chemicals in A. scholaris and the ability of ursolic acid to enhance the activity of antibiotics can constitute a valuable group of therapeutic agents in the future. Expand
Biotransformation of pentacyclic terpene isolated from Alstonia scholaris (R.BR.)
Abstract Betulin (1) a pentacyclic triterpene was isolated from medicinal plant Alstonia scholaris (R.BR.) and its structural modification by five filamentous fungi was investigated using flask shakeExpand
Anti-Proliferative Activity of Triterpenoids and Sterols Isolated from Alstonia scholaris against Non-Small-Cell Lung Carcinoma Cells
The ability of ursolic acid, Betulinic acid, betulin, and 2β,3β,28-lup-20(29)-ene-triol to inhibit the proliferative activity of NSCLC can constitute a valuable group of therapeutic agents in the future. Expand
Development and Validation of a High-Performance Liquid Chromatography Method for Standardization of the Bioactive Ethyl Acetate Fraction of Alstonia scholaris (Linn.) R. Br. Growing in Egypt
A validated reversed phase-high-performance liquid chromatography (RP-HPLC) method was developed for the standardization of the bio active ethyl acetate fraction and was shown to be simple, rapid, precise, reproducible, robust, and accurate for quality assessment of theBioactive fraction. Expand
Monoterpenoid indole alkaloids from the leaves of Alstonia scholaris and their NF-κB inhibitory activity.
Four new monoterpenoid indole alkaloids (MIAs), scholarisines P-S (1-4), and 14 known MIAs (5-18) were isolated from the leaves of Alstonia scholaris and exhibited significant NF-κB inhibitory activity. Expand
Pharmaceutical perspective on bioactives from Alstonia scholaris: ethnomedicinal knowledge, phytochemistry, clinical status, patent space, and future directions
The gap between the existing knowledge and the requisites for commercialization of A. scholaris derived products is stipulated and the requirements to convert existed knowledge into finished products are revealed. Expand
The Role of Pentacyclic Triterpenoids in the Allelopathic Effects of Alstonia scholaris
The results indicate that A. scholaris pentacyclic triterpenoids influence the growth of neighboring weeds by inhibiting seed germination, radicle growth, and functioning of photosystem II. Expand
Betulin and betulinic acid: triterpenoids derivatives with a powerful biological potential
Betulin, a pentacyclic triterpene and a plant pentacyclic triterpene metabolite, can be found in large quantities in the outer bark of the birches (Betula, Betulaceae). Betulinic acid, obtained byExpand
Alstonia scholaris (L.) R. Br. and Alstonia macrophylla Wall. ex G. Don: A comparative review on traditional uses, phytochemistry and pharmacology.
Comparison data about traditional uses, phytochemistry, pharmacology and toxicity of Alstonia scholaris and AlSTONia macrophylla is discussed and comparative data about in vitro and in vivo biological and pharmacological activities were promising. Expand
Ultrastructural response of wheat (Triticumaestivum L.) lines to potential allelopathy of Alstoniascholaris (L.) R. Br. leaf extract
A low concentration (15%) of allelochemical extract promoted growth and development and a high concentration (30%) was toxic, causing growth retardation and tissue damage. Expand


Terpens from aerial parts of Euphorbia splendida.
Euphorbia splendida Mobayen (Euphorbiaceae) is one of the Euphorbia species that has not been studied so far. The aerial parts of this plant was collected from Arak, Iran and extracted with methanolExpand
Glycosidation of lupane-type triterpenoids as potent in vitro cytotoxic agents.
3-O-glycosides of lupane-type triterpenoids represent an interesting class of potent in vitro cytotoxic agents and show that cancer cell lines are 8- to 12-fold more sensitive to the 3- O-alpha-L-rhamnopyranoside derivative of betulinic acid than the healthy cells. Expand
Evaluation of anticancer activity of the alkaloid fraction of Alstonia scholaris (Sapthaparna) in vitro and in vivo
The anticancer effect of various doses of an alkaloid fraction of Sapthaparna, Alstonia scholaris (ASERS), was studied in vitro in cultured human neoplastic cell lines and in Ehrlich ascites carcinoma bearing mice. Expand
Activity of extracts and alkaloids of thai Alstonia species against human lung cancer cell lines.
Methanol extracts of root barks of Alstonia macrophylla, A. glaucescens, and A. scholaris have been assessed for cytotoxic activity against two human lung cancer cell lines, MOR-P (adenocarcinoma) and COR-L23 (large cell carcinoma), using the SRB assay. Expand
Chemical Constituents of Dichloromethane Extract of Cultivated Satureja khuzistanica
Four compounds β-sitosterol, β-sitosterol-3-O-β-d-glucopyranoside, ursolic acid and 4′,5,6-trihydroxy-3′, 7-dimethoxyflavone were characterized from the dichloromethane extract of the aerial parts ofExpand
In Vitro Antioxidant and Free Radical Scavenging Activity of Alstonia scholaris Linn. R.Br.
The present work is carried out to study the antioxidant potential of ethanolic extract of Alstonia scholaris Linn. ( Apocynaceae ) using various in vitro tests includingExpand
Lupane and oleanane triterpenoids from the cones of Liquidamber styraciflua.
A new lupane- and a new oleanane-type (2) triterpenoid, together with a known compound, massagenic acid G, were isolated from the cones of Liquidamber styraciflua and evaluated for cytotoxicity against the P388 and A549 cell lines. Expand
Betulinic acid and its derivatives: a review on their biological properties.
A survey of the literature dealing with betulinic acid related biological properties that has appeared from the 1990's to the beginning of 2003 is reported. Expand
Evaluation of Alstonia scholaris leaves for broncho-vasodilatory activity.
Results coupled with the in vivo effects of ethanol extract reveal that the Alstonia scholaris leaves possess broncho-vasodilatory activity mediated presumably by prostaglandins, calcium antagonism and endothelium-derived relaxing factor(s), indicating that bronchodilation is not due to the direct tracheal smooth muscle relaxation. Expand
Identification and quantification of betulinic acid
Betulinic acid was identified in the bark and in the wood of Platanus acerifolia (plane) and of Betula pendula (white birch) by thin layer chromatography, gas chromatography (GC), GC coupled withExpand