Biogeochemistry: Methane and microbes

  title={Biogeochemistry: Methane and microbes},
  author={Rudolf K Thauer and Seigo Shima},
Microorganisms can carry out a wonderful range of chemical transformations. The anaerobic oxidation of methane seemed not to be among them. But it is — both with sulphate, and now it turns out, with nitrate.Ridding the world of methaneAlthough much speculated on, no microorganisms had been shown capable of anaerobic methane oxidation using nitrate as the sole electron acceptor. Now this reaction has been demonstrated in the laboratory in a microbial community with two members, one a slow… Expand
Nitrite-Dependent Anaerobic Methane-Oxidising Bacteria: Unique Microorganisms with Special Properties
A systematic review in the microbiological properties of M. oxyfera, especially for its special properties, and a new intra-aerobic pathway was discovered in M. Oxyfera that has not been found in other bacteria yet. Expand
Microbiology, ecology, and application of the nitrite-dependent anaerobic methane oxidation process
This mini-review summarizes the peculiar microbiology of M. oxyfera and discusses the potential ecological importance and engineering application of the n-damo process. Expand
Nitrite-dependent methane oxidation
Anaerobic methane oxidation coupled to denitrification was recently assigned to bacteria belonging to the uncultured phylum NC10. In this study, we incubated sediment from a eutrophic ditch harboringExpand
Methanogenic archaea: ecologically relevant differences in energy conservation
In methanogens with cytochromes, the first and last steps in methanogenesis from CO2 are coupled chemiosmotically, whereas in methenogens without cyto Chromes, these steps are energetically coupled by a cytoplasmic enzyme complex that mediates flavin-based electron bifurcation. Expand
Life close to the thermodynamic limit: how methanogenic archaea conserve energy.
This review will summarize the current knowledge of energy conservation of methanogens and focus on recent insights into structure and function of ion translocating enzymes found in these organisms. Expand
Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea.
The results show that bacteria can couple the anaerobic oxidation of methane to denitrification without the involvement of Archaea. Expand
Methane as Fuel for Anaerobic Microorganisms
There is evidence that anaerobic methane oxidation with sulfate proceeds at least in part via reversed methanogenesis involving the nickel enzyme methyl‐coenzyme M reductase for methane activation, which under standard conditions is an endergonic reaction, and thus inherently slow. Expand
Distribution and environmental significance of nitrite-dependent anaerobic methane-oxidising bacteria in natural ecosystems
Current knowledge of the ecological distribution of M. oxyfera-like bacteria and the potential importance of the N-DAMO process in reducing methane emissions in various natural ecosystems are summarized and the could become more important in the future. Expand
Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?
Abstract. Despite a large body of literature on microbial anaerobic oxidation of methane (AOM) in marine sediments and saline waters and its importance to the global methane (CH4) cycle, untilExpand
Evidence for nitrite-dependent anaerobic methane oxidation as a previously overlooked microbial methane sink in wetlands
  • B. Hu, Lidong Shen, +10 authors Y. He
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 2014
Previously unidentified direct evidence for the nitrite-dependent anaerobic methane oxidation (n-damo) process as a previously overlooked microbial methane sink in wetlands is provided by stable isotope measurements, quantitative PCR assays, and 16S rRNA and particulate methane monooxygenase gene clone library analyses. Expand


A microbial consortium couples anaerobic methane oxidation to denitrification
It is shown that the direct, anaerobic oxidation of methane coupled to denitrification of nitrate is possible and that the reaction presented here may make a substantial contribution to biological methane and nitrogen cycles. Expand
A marine microbial consortium apparently mediating anaerobic oxidation of methane
A large fraction of globally produced methane is converted to CO2 by anaerobic oxidation in marine sediments. Strong geochemical evidence for net methane consumption in anoxic sediments is based onExpand
A conspicuous nickel protein in microbial mats that oxidize methane anaerobically
The abundance of the nickel protein (7% of extracted proteins) in the mat suggests an important role in AOM, and similarities to methyl-coenzyme M reductase from methanogenic archaea are revealed. Expand
New perspectives on anaerobic methane oxidation.
A phylogenetic studies indicate that only specific groups of Archaea and SRB are involved in methane oxidation, and lipid biomarker studies detected isotopically depleted archaeal and bacterial biomarkers from marine methane vents, and indicate that Archaea are the primary consumers of methane. Expand
Methyl-coenzyme M reductase and the anaerobic oxidation of methane in methanotrophic Archaea.
The DNA from the methanotrophic Archaea has been shown to harbor gene homologues for methyl-coenzyme M reductase, which in methanogenic Archaea catalyses the methane-forming reaction. Expand
Identification of Methyl Coenzyme M Reductase A (mcrA) Genes Associated with Methane-Oxidizing Archaea
The existence and genomic conservation of methyl coenzyme M reductase (MCR), the enzyme catalyzing the terminal step in methanogenesis, was studied in AN ME-1 and ANME-2 archaea isolated from various marine environments, providing a basis for identifying methanotrophic archaea with mcrA sequences and defining a functional genomic link between methanogenic and methanosynthetic archaea. Expand
Anaerobic methane oxidation in a landfill-leachate plume.
Hydrochemical data suggest that a sulfate reducer-methanogen consortium may mediate this methane oxidation in the alluvial aquifer adjacent to Norman Landfill, OK, and demonstrate that natural attenuation through anaerobic methane oxidation can be an important sink for landfill methane in aquifer systems. Expand