Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to CYS-82 OF beta-phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002.

@article{Saune2008BiogenesisOP,
  title={Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to CYS-82 OF beta-phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002.},
  author={Nicolle A Saun{\'e}e and Shervonda R Williams and Donald A Bryant and Wendy M. Schluchter},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 12},
  pages={7513-22}
}
The Synechococcus sp. PCC 7002 genome encodes three genes, denoted cpcS-I, cpcU, cpcV, with sequence similarity to cpeS. CpcS-I copurified with His(6)-tagged (HT) CpcU as a heterodimer, CpcSU. When CpcSU was assayed for bilin lyase activity in vitro with phycocyanobilin (PCB) and apophycocyanin, the reaction product had an absorbance maximum of 622 nm and was highly fluorescent (lambda(max) = 643 nm). In control reactions with PCB and apophycocyanin, the products had absorption maxima at 635 nm… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

Biliprotein maturation: the chromophore attachment

Molecular microbiology • 2008
View 13 Excerpts
Highly Influenced

Structures and enzymatic mechanisms of phycobiliprotein lyases CpcE/F and PecE/F.

Proceedings of the National Academy of Sciences of the United States of America • 2017
View 1 Excerpt

References

Publications referenced by this paper.
Showing 1-7 of 7 references

Identification and Characterization of Enzymes Involved in Post-translational Modifications of Phycobiliproteins in the Cyanobacterium Synechocystis sp

C. A. Miller
PCC 6803. M. Sc. thesis, • 2007

The Characterization of Photosystem I and Ferredoxin-NADP Oxidoreductase in the Cyanobacterium Synechococcus sp. PCC 7002

W. M. Schluchter
Ph. D. thesis, • 1994

Mutational Analysis of the Genes Encoding Phycobilisome Components in the Cyanobacterium Synechococcus sp. PCC 7002

J. Zhou
Ph. D. thesis, • 1992

Cell Culture and Somatic Cell Genetics of Plants, Vol. 7B: The molecular biology of chloroplasts and mitochondria

D. A. Bryant
1991

Light-Energy Transduction in Photosynthesis: Higher Plant and Bacterial Models (Stevens

D. A. Bryant
American Society of Plant Physiologists, • 1988

FOOTNOTES *This research

W.M.S. MCB, B toD.A.
1974

Similar Papers

Loading similar papers…