Biogenesis of T pili in Agrobacterium tumefaciens requires precise VirB2 propilin cleavage and cyclization.

@article{Lai2002BiogenesisOT,
  title={Biogenesis of T pili in Agrobacterium tumefaciens requires precise VirB2 propilin cleavage and cyclization.},
  author={E M Lai and Ralf Eisenbrandt and Markus Kalkum and Erich Lanka and Clarence I. Kado},
  journal={Journal of bacteriology},
  year={2002},
  volume={184 1},
  pages={327-30}
}
VirB2 propilin is processed by the removal of a 47-amino-acid signal peptide to generate a 74-amino-acid peptide product in both Escherichia coli and Agrobacterium tumefaciens. The cleaved VirB2 protein is further cyclized to form the T pilin in A. tumefaciens but not in E. coli. Mutations in the signal peptidase cleavage sequence of VirB2 propilin cause the formation of aberrant T pilin and also severely attenuate virulence. No T pilus was observed in these mutants. The potential role of the… CONTINUE READING

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