Biofilm formation by Streptococcus pneumoniae: role of choline, extracellular DNA, and capsular polysaccharide in microbial accretion.

@article{Moscoso2006BiofilmFB,
  title={Biofilm formation by Streptococcus pneumoniae: role of choline, extracellular DNA, and capsular polysaccharide in microbial accretion.},
  author={Miriam Moscoso and Ernesto Garcia and R. Villarroel Lopez},
  journal={Journal of bacteriology},
  year={2006},
  volume={188 22},
  pages={
          7785-95
        }
}
Streptococcus pneumoniae colonizes the human upper respiratory tract, and this asymptomatic colonization is known to precede pneumococcal disease. In this report, chemically defined and semisynthetic media were used to identify the initial steps of biofilm formation by pneumococcus during growth on abiotic surfaces such as polystyrene or glass. Unencapsulated pneumococci adhered to abiotic surfaces and formed a three-dimensional structure about 25 microm deep, as observed by confocal laser… CONTINUE READING
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