Biofilm formation by Moraxella catarrhalis in vitro: roles of the UspA1 adhesin and the Hag hemagglutinin.

@article{Pearson2006BiofilmFB,
  title={Biofilm formation by Moraxella catarrhalis in vitro: roles of the UspA1 adhesin and the Hag hemagglutinin.},
  author={Melanie M Pearson and Cassie A Laurence and Sarah E Guinn and Eric J. Hansen},
  journal={Infection and immunity},
  year={2006},
  volume={74 3},
  pages={1588-96}
}
Mutant analysis was used to identify Moraxella catarrhalis gene products necessary for biofilm development in a crystal violet-based assay involving 24-well tissue culture plates. The wild-type M. catarrhalis strains that formed the most extensive biofilms in this system proved to be refractory to transposon mutagenesis, so an M. catarrhalis strain was constructed that was both able to form biofilms in vitro and amenable to transposon mutagenesis. Chromosomal DNA from the biofilm-positive… CONTINUE READING