Biofilm and capsule formation of the diatom Achnanthidium minutissimum are affected by a bacterium

  title={Biofilm and capsule formation of the diatom Achnanthidium minutissimum are affected by a bacterium},
  author={Miriam Windler and Katrin Leinweber and Carolina Rio Bartulos and Bodo Philipp and Peter G. Kroth},
  journal={Journal of Phycology},
Photoautotrophic biofilms play an important role in various aquatic habitats and are composed of prokaryotic and/or eukaryotic organisms embedded in extracellular polymeric substances (EPS). We have isolated diatoms as well as bacteria from freshwater biofilms to study organismal interactions between representative isolates. We found that bacteria have a strong impact on the biofilm formation of the pennate diatom Achnanthidium minutissimum. This alga produces extracellular capsules of… 
Cell-bound exopolysaccharides from an axenic culture of the intertidal mudflat Navicula phyllepta diatom affect biofilm formation by benthic bacteria
This work points out the complexity of the benthic natural biofilms with specific microalgae/bacteria interactions and underlines the possibility to use axenic diatoms as a source of bioactive compounds.
Capsules of the diatom Achnanthidium minutissimum arise from fibrillar precursors and foster attachment of bacteria
It is shown that bacteria preferentially attach to capsules, instead of non-encapsulated A. minutissimum cells, which supports the idea that capsules mediate diatom-bacteria interactions.
The Alterations of Biofilm Formation and EPS Characteristics of a Diatom by a Sponge-Associated Bacterium Psychrobacter sp.
It is proposed that changes in monosaccharides composition of the EPS of Stauroneis sp.
Enhanced biofilm formation aids adaptation to extreme warming and environmental instability in the diatom Thalassiosira pseudonana and its associated bacteria
  • C. Schaum
  • Environmental Science
    Limnology and Oceanography
  • 2018
Recent studies have highlighted the capacity of marine diatoms at the foundation of ocean food webs and biogeochemical cycles to respond to environmental change. These responses are a combination of
Exploring the microbiome of the “star” freshwater diatom Asterionella formosa in a laboratory context
A laboratory model of intermediate complexity between complex natural communities and laboratory pure culture models is established and the functional architecture of the bacterial community associated with A. formosa is explored, bringing insights into the bacterial diversity found in diatom-bacterial communities.
Antibiofilm Activity of a Trichoderma Metabolite against Xanthomonas campestris pv. campestris, Alone and in Association with a Phage
It is demonstrated that Xccφ1, alone or in combination with 6PP and HA, interfered with the gene pathways involved in the formation of biofilm and can be used as a model for other biofilm-producing bacteria.
Biotechnological Aspects of Extracellular Polysaccharide Production by Microalga Netrium digitus using Twin-Layer
Fundamentals for immobilized culture of EPS producing microalgae are presented as well as suitable culturing conditions to increase production, and the introduction of an adapted spectrophotometric method for EPS quantification in culture supernatants using the inorganic dye Ruthenium Red is presented.


Purification of benthic diatoms from associated bacteria using the antibiotic imipenem
A short and feasible protocol for culture axenification was successfully applied to cultures of the benthic diatoms Achnanthidium minutissimum, Cymbella affiniformis and Nitzschia palea and might also be useful for the purification of other cultivated diatom strains.
Diatom-associated bacteria are required for aggregation of Thalassiosira weissflogii
Interactions between heterotrophic bacteria and diatoms increased aggregate formation and particle sinking and thus may enhance the efficiency of the biological pump.
Principal component analysis indicates a correlation between carbohydrate composition of EPS fractions and the phylogenetic relationship of the respective species, indicating that EPS analyses under defined culture conditions may support taxonomic analyses.
Role of bacteria and bacterial exopolymer in the attachment of Achnanthes longipes (bacillariophyceae)
The attachment of diatoms to surfaces is an important and poorly understood step in the development of biofouling communities and the influence of the base material and bacterial conditioning on diatom attachment was determined.
Bacteria Associated with Benthic Diatoms from Lake Constance: Phylogeny and Influences on Diatom Growth and Secretion of Extracellular Polymeric Substances
Phylogenetic analysis and coculture studies indicate an adaptation of Proteobacteria and Bacteroidetes to the microenvironment created by the diatom biofilm, with a clear dominance of Alphaproteobacteria.
Physical characterization and diel dynamics of different fractions of extracellular polysaccharides in an axenic culture of a benthic diatom
The excretion of extracellular polymeric substances (EPS) by an axenic culture of the benthic diatom Cylindrotheca closterium was investigated. Two sequential extraction steps proved to be suficient
Isolation and characterization of extracellular polysaccharides from the epipelic diatoms Cylindrotheca closterium and Navicula salinarum
Investigation of the production and composition of extracellular polymeric substances (EPS) in axenic batch cultures of the benthic marine epipelic diatoms Navicula salinarum and Cylindrotheca closterium found that glucose and xylose were the main constituents, but several other monosaccharides were present in smaller quantities.
Adhesion of bacteria and diatoms to surfaces in the sea : a review
Genetic evidence now supports the idea that surface proximity promotes the induction of new metabolic capabilities in microorganisms, however the environmental signal responsible for this is not agreed and it is proposed that restricted diffusion at a surface plays a role in the process.
Parasitic growth of Pseudomonas aeruginosa in co-culture with the chitinolytic bacterium Aeromonas hydrophila.
P. aeruginosa could profit from a substrate that was originally not bioavailable to it by influencing the metabolism and viability of A. hydrophila in a parasitic way with chitin as carbon, nitrogen and energy source.
Algae-bacteria interactions and their effects on aggregation and organic matter flux in the sea.
The hypothesis that algae-bacteria interactions are instrumental in aggregate dynamics and organic matter flux is tested and highlights the importance of heterotrophic bacteria to control the development and aggregation of phytoplankton in marine systems.