Bioenergetics and solute transport in lactococci.

  title={Bioenergetics and solute transport in lactococci.},
  author={Wilhelmus Nicolaas Konings and Bert Poolman and Arnold J.M. Driessen},
  journal={Critical reviews in microbiology},
  volume={16 6},
During the last few years the studies about the physiology and bioenergetics of lactic acid bacteria during growth and starvation have evolved from a descriptive level to an analysis of the molecular events in the regulation of various processes. Considerable progress has been made in the understanding of the modes of metabolic energy generation, the mechanism of homeostasis of the internal pH, and the mechanism and regulatory processes of transport systems for sugars, amino acids, peptides… 

Energy transduction in lactic acid bacteria.

This work not only includes the biochemistry of the enzymes and the bioenergetics of the processes, but also the genetics of the genes encoding the energy transducing proteins.

pH Homeostasis in Lactlc Acld Bacterial

The means by which lactic acid bacteria defend against low intracellular pH are presented, and particular attention is devoted to the proton-translocating ATPase, an enzyme that is largely responsible for pH homeostasis in fermentative lactic Acid bacteria.

Physiology of pyruvate metabolism in Lactococcus lactis

It will be demonstrated that the metabolic transformation of pyruvate can be predicted if the growth-limiting constraints are adequately established and the normal pathways can no longer maintain balanced carbon flux.

Secondary transport of amino acids by membrane vesicles derived from lactic acid bacteria

A membrane-fusion procedure has been developed by which pmf-generating systems can be functionally incorporated into the bacterial membrane and this improved model system has been used to analyze the properties of amino acid transport systems in lactococci.

The proteotytic systems of lactic acid bacteria

A model for the proteolytic pathway is proposed that consists of an extracellularly located serine-proteinase, transport systems specific for di-tripeptides and oligopeptides, and a multitude of intracellular peptidases.

The cell membrane and the struggle for life of lactic acid bacteria.

The major life-threatening event for lactic acid bacteria (LAB) in their natural environment is the depletion of their energy sources and LAB can survive such conditions only for a short period of

The cell membrane and the struggle for life of lactic acid bacteria

The major life-threatening event for lactic acid bacteria (LAB) in their natural environment is the depletion of their energy sources and LAB can survive such conditions only for a short period of

Solute transport and energy transduction in bacteria

An overview is given of the various secondary transport processes that form ion-gradients at the expense of precursor (substrate) and/or end-product concentration gradients.

Microbial transport: Adaptations to natural environments

The cytoplasmic membrane of bacteria is the matrix for metabolic energy transducing processes such as proton motive force generation and solute transport, and several transport systems are specifically aimed at the generation of metabolic energy during periods of energy-limitation.

Physiologie des bactéries du genre Lactococcus en conditions de privation nutritionnelle. Une revue

The modifications of the overall cell metabolism during starvation essentially concerned regulation of carbohydrates metabolism with the enzymes involved in glycolysis brought about by changes in intracellular effector levels.



Role of Metabolic Energy in the Transport of β-Galactosides by Streptococcus lactis

Evidence that the transport carriers were functional in the absence of glucose or in the presence of uncoupling agents included the demonstration of counterflow, which depends on competitive inhibition for the carrier for exit.

Branched-chain amino acid transport in Streptococcus agalactiae

  • J. W. Moran
  • Biology
    Applied and environmental microbiology
  • 1980
The transport of the branched-chain amino acids in Streptococcus agalactiae was characterized and energy was found to be coupled to transport by the action of adenosine triphosphatase and the generation of a proton motive force.

Regulation of sugar transport and metabolism in lactic acid bacteria

It has been established that PEP provides the crucial link between transport and energy-yielding reactions of the PTS: glycolysis cycle, and that both ATP-dependent glucokinase and PEP-dependent mannose-PTS can participate in the phosphorylation of intracellular glucose.

Regulation of the glutamate-glutamine transport system by intracellular pH in Streptococcus lactis.

The dependence of glutamate transport on the accumulation of potassium ions in potassium-filled and -depleted cells is caused by the regulation of intracellular pH by potassium movement.

Regulation of cytoplasmic pH in bacteria.

  • I. Booth
  • Biology
    Microbiological reviews
  • 1985
The aim of this review of pH homeostasis in bacteria is to assess the role of transport systems in the regulation of cytoplasmic pH and to consider the principal questions which remain to be answered.


The uptake of citrate by Streptococcus diacetilactis is mediated by a transport system that was distinguished from passive diffusion by inducibility and kinetics of uptake, and in these characteristics the system is similar to the beta-galactoside permease of Escherichia coli.

Active transport of L-valine by Streptococcus diacetilactis.

Transport of l-valine by resting cells of Streptococcus diacetilactis strain 18-16 was sensitive to changes in temperature resulting in a temperature coefficient of about 1.8, indicating the probable involvement of metabolically produced energy.

Proton-coupled accumulation of galactoside in Streptococcus lactis 7962.

  • E. R. KashketT. Wilson
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1973
The protonmotive force, calculated from the membrane potential and the pH gradient, was found to be directly related to the accumulation of galactoside, in accordance with the chemiosmotic hypothesis.

Mechanism and energetics of dipeptide transport in membrane vesicles of Lactococcus lactis

Results indicate that the Ala-Glu transport system has a broad substrate specificity and is directly coupled to the proton motive force as a driving force.