Biodiversity and biogeography of phages in modern stromatolites and thrombolites.

Abstract

Viruses, and more particularly phages (viruses that infect bacteria), represent one of the most abundant living entities in aquatic and terrestrial environments. The biogeography of phages has only recently been investigated and so far reveals a cosmopolitan distribution of phage genetic material (or genotypes). Here we address this cosmopolitan distribution through the analysis of phage communities in modern microbialites, the living representatives of one of the most ancient life forms on Earth. On the basis of a comparative metagenomic analysis of viral communities associated with marine (Highborne Cay, Bahamas) and freshwater (Pozas Azules II and Rio Mesquites, Mexico) microbialites, we show that some phage genotypes are geographically restricted. The high percentage of unknown sequences recovered from the three metagenomes (>97%), the low percentage similarities with sequences from other environmental viral (n = 42) and microbial (n = 36) metagenomes, and the absence of viral genotypes shared among microbialites indicate that viruses are genetically unique in these environments. Identifiable sequences in the Highborne Cay metagenome were dominated by single-stranded DNA microphages that were not detected in any other samples examined, including sea water, fresh water, sediment, terrestrial, extreme, metazoan-associated and marine microbial mats. Finally, a marine signature was present in the phage community of the Pozas Azules II microbialites, even though this environment has not been in contact with the ocean for tens of millions of years. Taken together, these results prove that viruses in modern microbialites display biogeographical variability and suggest that they may be derived from an ancient community.

DOI: 10.1038/nature06735

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@article{Desnues2008BiodiversityAB, title={Biodiversity and biogeography of phages in modern stromatolites and thrombolites.}, author={Christelle Desnues and Beltran Rodriguez-Brito and Steve Rayhawk and Scott Kelley and Tuong Xuan Tran and Matthew Haynes and Hong Liu and Mike Furlan and Linda Wegley and Betty Chau and Yijun Ruan and Dana L. Hall and Florent E. Angly and Robert A. Edwards and Linlin Li and Rebecca L. Vega Thurber and Ruth Pamela Reid and Janet L. Siefert and Val{\'e}ria Helena Salgado Souza and David L Valentine and Brandon K. Swan and Mya Breitbart and Forest Rohwer}, journal={Nature}, year={2008}, volume={452 7185}, pages={340-3} }