Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs

@article{Bragonzi2000BiodistributionAT,
  title={Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs},
  author={Alessandra Bragonzi and Giorgia Dina and Ana M. D{\'i}az Villa and Giliola Calori and Alessandra Biffi and Claudio Bordignon and Baroukh Maurice Assael and Massimo Conese},
  journal={Gene Therapy},
  year={2000},
  volume={7},
  pages={1753-1760}
}
Biodistribution of nonviral cationic vector/DNA complexes was studied after systemic or intratracheal administration to the lungs and correlated with transgene expression. Intravenous injection in C57Bl/6 mice gave maximal and significant luciferase expression in the lungs with the cationic polymer PEI 22K/DNA complexes at the highest ratios of positive/negative charges versus DNA alone. While DOTAP/DNA complexes with high charge ratio determined lower but still significant luciferase activity… CONTINUE READING