Biodistribution and retention of locally administered human mesenchymal stromal cells: Quantitative polymerase chain reaction-based detection of human DNA in murine organs.

@article{Creane2017BiodistributionAR,
  title={Biodistribution and retention of locally administered human mesenchymal stromal cells: Quantitative polymerase chain reaction-based detection of human DNA in murine organs.},
  author={Michael Creane and Linda Howard and Timothy O'Brien and Cynthia M. Coleman},
  journal={Cytotherapy},
  year={2017},
  volume={19 3},
  pages={
          384-394
        }
}
BACKGROUND Determining the distributive fate and retention of a cell therapy product after administration is an essential part of characterizing it's biosafety profile. Therefore, regulatory guidelines stipulate that biodistribution assays are a requirement prior to advancing a cell therapy to the clinic. Here the development of a highly sensitive quantitative polymerase chain reaction (qPCR)-based method of tracking the biodistribution and retention of human mesenchymal stromal cells (hMSCs… CONTINUE READING