Biochemistry: A cadmium enzyme from a marine diatom

  title={Biochemistry: A cadmium enzyme from a marine diatom},
  author={T. Lane and M. Saito and G. George and I. Pickering and R. Prince and F. Morel},
The ocean biota contains a vast reservoir of genomic diversity. Here we present the sequence and preliminary characterization of a protein that is a cadmium-containing carbonic anhydrase from the marine diatom Thalassiosira weissflogii. The existence of a cadmium enzyme in marine phytoplankton may indicate that there is a unique selection pressure for metalloenzymes in the marine environment, and our discovery provides a long-awaited explanation for the nutrient-like behaviour of cadmium in the… Expand
Cadmium-Containing Carbonic Anhydrase CDCA1 in Marine Diatom Thalassiosira weissflogii
The biochemical and structural features of CDCA1 enzyme will be presented together with its putative biotechnological applications for the detection of metal ions in seawaters. Expand
Diversity of the cadmium-containing carbonic anhydrase in marine diatoms and natural waters.
It is shown that CDCA is widespread in diatom species and ubiquitous in the environment, and the cdca primers developed in this study should be useful for detecting cdca genes in the field, and studying the conditions under which they are expressed. Expand
Prokaryotic carbonic anhydrases of Earth's environment.
The physiology and biochemistry of carbonic anhydrases from prokaryotes belonging to the domains Bacteria and Archaea that play key roles in the ecology of Earth's biosphere are focused on. Expand
CDCA1 From Thalassiosira weissflogii as Representative Member of ζ-Class CAs: General Features and Biotechnological Applications
The high affinity of CDCA1 active site for cadmium makes this enzyme a good candidate for the development of CA-based biosensors for determination of free metal ions in the seawater, and potential biotechnological applications in CO2 capture processes may also be envisaged. Expand
Expression of cadmium carbonic anhydrase of diatoms in seawater
CDCA is indeed expressed in ocean waters and is regulated in a manner consistent with its putative role in CO 2 acquisition, and a high level of CDCA expression was found in samples from the Equatorial Pacific and coastal New Jersey. Expand
Expression and regulation of carbonic anhydrases in the marine diatom Thalassiosira pseudonana and in natural phytoplankton assemblages from Great Bay, New Jersey.
Investigation of expressed sequence tag libraries revealed putative homologs of the archetypal diatom delta-carbonic anhydrase TWCA1 in a broad range of eukaryotic phytoplankton including haptophytes, prasinophytes and dinoflagellates, indicating that this CA is expressed under a broadrange of environmental conditions and not restricted to low pCO(2) and low Zn. Expand
Structure and metal exchange in the cadmium carbonic anhydrase of marine diatoms
Despite lack of sequence homology, CDCA is a structural mimic of a functional β-carbonic anhydrase dimer, with striking similarity in the spatial organization of the active site residues. Expand
Structural and inhibition insights into carbonic anhydrase CDCA1 from the marine diatom Thalassiosira weissflogii.
The characterization of zeta-CA, a novel enzyme in the marine diatom Thalassiosira weissflogii, which consists of three repeats, has been completed, reporting the X-ray structure of the last repeat, CDCA1-R3 in its cadmium-bound form, and presenting a model of the full length protein obtained by docking approaches. Expand
An External d-Carbonic Anhydrase in a Free-Living Marine Dinoflagellate May Circumvent Diffusion-Limited Carbon Acquisition 1 [ W ]
The oceans globally constitute an important sink for carbon dioxide (CO2) due to phytoplankton photosynthesis. However, the marine environment imposes serious restraints to carbon fixation. First,Expand
A new widespread subclass of carbonic anhydrase in marine phytoplankton
Different lines of evidence are presented showing that the protein LCIP63, identified in the marine diatom Thalassiosira pseudonana, is a CA, but sequence analysis showed that it has a low identity with any known CA and therefore belongs to a new subclass that the authors designate as iota-CA. Expand


A biological function for cadmium in marine diatoms.
  • T. Lane, F. M. Morel
  • Chemistry, Biology
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2000
Evidence is provided of a biological role for Cd in the marine diatom Thalassiosira weissflogii under conditions of low zinc, typical of the marine environment, and of a Cd-specific CA which can replace the Zn enzyme TWCA1 in its carbon-concentrating mechanism. Expand
A Novel Evolutionary Lineage of Carbonic Anhydrase (ε Class) Is a Component of the Carboxysome Shell
It is reported that the carboxysomal shell protein, CsoS3, from Halothiobacillus neapolitanus is a novel carbonic anhydrase (epsilon-class CA) that has an evolutionary lineage distinct from those previously recognized in animals, plants, and other prokaryotes. Expand
The bioinorganic chemistry of the ancient ocean: the co-evolution of cyanobacterial metal requirements and biogeochemical cycles at the Archean–Proterozoic boundary?
Abstract Recent evidence from the sulfur isotopic record indicates a transition ∼2.5 billion years ago from an ocean chemistry first dominated by iron and then by sulfide. It has been hypothesizedExpand
Prokaryotic carbonic anhydrases.
Recent work has shown that carbonic anhydrase is widespread in metabolically diverse species from both the Archaea and Bacteria domains indicating that the enzyme has a more extensive and fundamental role in prokaryotic biology than previously recognized. Expand
Cadmium and cobalt substitution for zinc in a marine diatom
In sea water with low Zn concentration, mimicking conditions of the ocean surface waters, Cd stimulates the growth of the marine diatom Thalassiosira weissflogii by substituting for Zn in certain macromolecules, indicating that Co could be an important nutrient for algal growth for reasons other than its role in vitamin B12. Expand
Zinc and carbon co-limitation of marine phytoplankton
PROCESSES that control carbon uptake by marine phytoplankton are important in the global carbon cycle11–3. Uptake of CO2 itself may be limited by diffusion4. Bicarbonate uptake may be limited by zincExpand
On the marine geochemistry of cadmium
STUDIES of the geochemistry of trace metals in sea water are of great potential importance, given the wide diversity in their properties. Knowledge of their distribution provides information not onlyExpand
Environmental Genome Shotgun Sequencing of the Sargasso Sea
Over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors are identified, suggesting substantial oceanic microbial diversity. Expand
X-ray absorption spectroscopy of cadmium phytochelatin and model systems.
The Cd K-edge extended X-ray absorption fine structure (EXAFS) data, together with the CdK, LI, LII and LIII near-edge spectra, reveal a predominantly tetrahedral coordination of cadmium by sulfur in both the phytochelatin and synthetic peptide complexes. Expand
The active site architecture of Pisum sativum β‐carbonic anhydrase is a mirror image of that of α‐carbonic anhydrases
Despite differing folds, α‐ and β‐carbonic anhydrase have converged upon a very similar active site design and are likely to share a common mechanism. Expand