Biochemical sub-fractionation of the mammalian Golgi apparatus.


We have exploited the breakdown of the Golgi apparatus that occurs during mitosis to isolate subfractions using immuno-affinity methods. Rat liver Golgi stacks were treated with mitotic cytosol from HeLa cells, and the fragments were then incubated with antibodies immobilized on magnetic beads. Antibodies against the cis-Golgi marker, GM130, bound membranes… (More)


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