Biochemical study and in vitro insect immune suppression by a trypsin-like secreted protease from the nematode Steinernema carpocapsae.

@article{Balasubramanian2010BiochemicalSA,
  title={Biochemical study and in vitro insect immune suppression by a trypsin-like secreted protease from the nematode Steinernema carpocapsae.},
  author={Natesan Balasubramanian and Duarte Toubarro and N. J. O. Sim{\~o}es},
  journal={Parasite immunology},
  year={2010},
  volume={32 3},
  pages={165-75}
}
A trypsin-like serine protease was purified by gel filtration and anion-exchange chromatography from the excretory-secretory products of parasitic phase Steinernema carpocapsae. The purified protease exhibited a molecular mass of about 29 kDa by SDS-PAGE and displayed a pI of 6.3. This protease exhibited high activity with trypsin-specific substrate N-Ben… CONTINUE READING