Biochemical route and control of butyrate synthesis in Butyribacterium methylotrophicum

@article{Shen1996BiochemicalRA,
  title={Biochemical route and control of butyrate synthesis in Butyribacterium methylotrophicum},
  author={G.-J. Shen and Bassam A. Annous and Robert W. Lovitt and Mahendra Kumar Jain and Joseph Gregory Zeikus},
  journal={Applied Microbiology and Biotechnology},
  year={1996},
  volume={45},
  pages={355-362}
}
 Butyribacterium methylotrophicum produced more butyrate when grown on lactate than when grown on glucose, and only acetate was detected during growth on pyruvate. Higher levels of NADH were found in butyrate-producing than in acetate-producing cells. The addition of neutral red, an electron-flow modulator, to cells growing on pyruvate altered the carbon and electron flow from acetate plus H2 synthesis to butyrate synthesis. Enzymatic analysis suggested that pyruvate was produced from glucose… CONTINUE READING

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