Biochemical mechanism of acetaminophen (APAP) induced toxicity in melanoma cell lines.

@article{Vad2009BiochemicalMO,
  title={Biochemical mechanism of acetaminophen (APAP) induced toxicity in melanoma cell lines.},
  author={Nikhil M. Vad and Garret L. Yount and Dan K. Moore and Jon Weidanz and Majid Y. Moridani},
  journal={Journal of pharmaceutical sciences},
  year={2009},
  volume={98 4},
  pages={1409-25}
}
In this work, we investigated the biochemical mechanism of acetaminophen (APAP) induced toxicity in SK-MEL-28 melanoma cells using tyrosinase enzyme as a molecular cancer therapeutic target. Our results showed that APAP was metabolized 87% by tyrosinase at 2 h incubation. AA and NADH, quinone reducing agents, were significantly depleted during APAP oxidation by tyrosinase. The IC(50) (48 h) of APAP towards SK-MEL-28, MeWo, SK-MEL-5, B16-F0, and B16-F10 melanoma cells was 100 microM whereas it… CONTINUE READING

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