Biochemical identification and phylogenetic relationships in free-living amoebas of the genus Naegleria.

  title={Biochemical identification and phylogenetic relationships in free-living amoebas of the genus Naegleria.},
  author={Pierre Pernin and Marie Cariou and Anouchka Jacquier},
  journal={The Journal of protozoology},
  volume={32 4},
Using isoelectric focusing, the zymograms of 23 pathogenic and nonpathogenic Naegleria strains were studied for the activity of 16 enzymes. Certain enzymes (lactate dehydrogenase, L-threonine dehydrogenase, superoxide dismutase, acid phosphatase, malic enzyme, and leucine aminopeptidase) proved particularly useful from a practical point of view as they allow easy and reliable identification of pathogenic N. fowleri and N. australiensis as well as nonpathogenic N. lovaniensis strains. Genetic… 
Application of isoenzymatic typing to the identification of nonaxenic strains ofNaegleria (Protozoa, Rhizopoda)
Isoenzymatic typing of the different species of Naegleria was studied by comparing isoelectric focusing on axenic and nonaxenic strains using the most discriminating enzymes: lactate dehydrogenase (LDH), malic enzyme (ME), β-HBDH, superoxide dismutase (SOD), and acid phosphatase (AP).
Determination of taxonomic status of pathogenic and nonpathogenic Entamoeba histolytica zymodemes using isoenzyme analysis.
  • D. Blanc
  • Medicine
    The Journal of protozoology
  • 1992
Genetic distance analysis clearly demonstrates the existence of two separate groups within the species E. histolytica, pathogenic and nonpathogenic.
Large genetic heterogeneity within amoebas of the species Naegleria gruberi and evolutionary affinities to the other species of the genus.
The allozyme survey was extended to 7 strains of Naegleria gruberi and N. jadini in order to further characterize the genetic structure of these free-living amoebas. As formerly known for several
Diversity of free-living ‘naked’ amoeboid organisms
The extent of diversity of these organisms has been recognized, as methods to detect, culture, characterize and identify them has increased and it is reasonable to anticipate that the current 40 000 species of protists will increase substantially as amoeboid organisms are cultivated from poorly accessible niches and from extreme environs.
A Century of Research on the Amoeboflagellate Genus Naegleria
The state of affairs concerning species designation based on phylogeny in the genus Acanthamoeba, another free-living amoeba with species pathogenic to man, is discussed and two lineages are discussed and identified as separate species.
A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis.
Biology of Naegleria spp
Six species of Naegleria characteristically undergo transformation from amoebae to flagellates and may be distinguished on the basis of cyst morphology, temperature tolerance, immunologic criteria, and isoenzyme patterns.
The amoeba-to-flagellate transformation test is not reliable for the diagnosis of the genus Naegleria. Description of three new Naegleria spp.
Failing to form flagellates since their isolation, even when different transformation procedures are used, are two Naegleria strains from Chile and Indonesia, which are proposed to represent new species.


Starch gel electrophoresis: an effective method for separation of pathogenic and nonpathogenic Naegleria strains.
Isoenzyme electrophoresis of 7 different enzyme systems was used to compare 24 strains of Naegleria fowleri and 6 strains of N. gruberi, finding that the 22 pathogenic strains constitute a homogenous species and the remaining nonpathogenic strains could be separated into 2 groups.
Temperature Tolerance of Pathogenic and Nonpathogenic Free-Living Amoebas
Within tested strains of the genera Naegleria and Acanthamoeba the ability to grow at high temperatures seems directly related to virulence, with nonvirulent strains unable to grow at normal or
Comparative Study of Six Strains of Naegleria with Special Reference to Nonpathogenic Variants of Naegleria fowleri
There exists an intermediate form between N. gruberi and N. fowleri, with a strong relationship to the latter species, and strains of Naegleria isolated from a body of water polluted with thermal effluents were compared.
Identification of Leishmania spp. by multiple isozyme analysis.
Calculations from the CAE data on average polymorphism indicated that Leishmania species/types or groups can be expected to be about 25% polymorphic, which suggests that isolate pairs which have profiles about 75% or more identical should be considered samples from the same species/type, and isolates that are significantly less than 75% identical are therefore samples from different species/ types.
Use of an axenic medium for differentiation between pathogenic and nonpathogenic Naegleria fowleri isolates.
Growth in an axenic medium composed by Chang allowed separation of pathogenic from nonpathogenic Naegleria fowleri strains, since only the former show luxuriant growth in this medium, and it was found that using this medium in the early stage of Naeglersia sampling favors isolation of pathogen strains in mixtures of Naegalia.
A genetic comparison between Brazilian and Bolivian zymodemes of Trypanosoma cruzi.
  • M. TibayrencM. Miles
  • Biology, Political Science
    Transactions of the Royal Society of Tropical Medicine and Hygiene
  • 1983
Allozymes as diagnostic characters of sibling species of Drosophila.
  • F. J. AyalaJ. Powell
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1972
Two groups of sibling species show that allozyme differences can be used as species-diagnostic characters and if several diagnostic loci are used, the species of any individual can be diagnosed with virtual certainty.
Genetic Distance between Populations
  • M. Nei
  • Biology
    The American Naturalist
  • 1972
If enough data are available, genetic distance between any pair of organisms can be measured in terms of D, and this measure is applicable to any kind of organism without regard to ploidy or mating scheme.