Biochemical identification and phylogenetic relationships in free-living amoebas of the genus Naegleria.

  title={Biochemical identification and phylogenetic relationships in free-living amoebas of the genus Naegleria.},
  author={Pierre Pernin and Marie Cariou and Anouchka Jacquier},
  journal={The Journal of protozoology},
  volume={32 4},
Using isoelectric focusing, the zymograms of 23 pathogenic and nonpathogenic Naegleria strains were studied for the activity of 16 enzymes. Certain enzymes (lactate dehydrogenase, L-threonine dehydrogenase, superoxide dismutase, acid phosphatase, malic enzyme, and leucine aminopeptidase) proved particularly useful from a practical point of view as they allow easy and reliable identification of pathogenic N. fowleri and N. australiensis as well as nonpathogenic N. lovaniensis strains. Genetic… 
Application of isoenzymatic typing to the identification of nonaxenic strains ofNaegleria (Protozoa, Rhizopoda)
Isoenzymatic typing of the different species of Naegleria was studied by comparing isoelectric focusing on axenic and nonaxenic strains using the most discriminating enzymes: lactate dehydrogenase (LDH), malic enzyme (ME), β-HBDH, superoxide dismutase (SOD), and acid phosphatase (AP).
Determination of taxonomic status of pathogenic and nonpathogenic Entamoeba histolytica zymodemes using isoenzyme analysis.
  • D. Blanc
  • Medicine
    The Journal of protozoology
  • 1992
Genetic distance analysis clearly demonstrates the existence of two separate groups within the species E. histolytica, pathogenic and nonpathogenic.
Zymogram patterns of Naegleria spp isolated from natural water sources in Taling Chan district, Bangkok.
The zymograms of the Naegleria fowleri strains were heterogenically different from the thermophilic 45 degrees C Naeglersia spp isolated from the environment.
Large genetic heterogeneity within amoebas of the species Naegleria gruberi and evolutionary affinities to the other species of the genus.
The allozyme survey was extended to 7 strains of Naegleria gruberi and N. jadini in order to further characterize the genetic structure of these free-living amoebas. As formerly known for several
Diversity of free-living ‘naked’ amoeboid organisms
The extent of diversity of these organisms has been recognized, as methods to detect, culture, characterize and identify them has increased and it is reasonable to anticipate that the current 40 000 species of protists will increase substantially as amoeboid organisms are cultivated from poorly accessible niches and from extreme environs.
A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis.
Biology of Naegleria spp
Six species of Naegleria characteristically undergo transformation from amoebae to flagellates and may be distinguished on the basis of cyst morphology, temperature tolerance, immunologic criteria, and isoenzyme patterns.
The amoeba-to-flagellate transformation test is not reliable for the diagnosis of the genus Naegleria. Description of three new Naegleria spp.
Failing to form flagellates since their isolation, even when different transformation procedures are used, are two Naegleria strains from Chile and Indonesia, which are proposed to represent new species.


The Biochemical Identification of Vahlkampfiid Amoebae1
Isoenyme electrophoresis of three different enzymes was used to compare 16 strains of vahlkampfiid amoebae and a strain identified as a slime mold, finding an isolate of Naegleria fowleri on the basis of zymogram type and other characters, confirming Cursons & Brown's similar conclusion drawn in 1975.
Starch gel electrophoresis: an effective method for separation of pathogenic and nonpathogenic Naegleria strains.
Isoenzyme electrophoresis of 7 different enzyme systems was used to compare 24 strains of Naegleria fowleri and 6 strains of N. gruberi, finding that the 22 pathogenic strains constitute a homogenous species and the remaining nonpathogenic strains could be separated into 2 groups.
Isolation and identification of pathogenic Naegleria australiensis (Amoebida, Vahlkampfiidae) from a spa in northern Italy
The virulence of the isolates for mice corresponded with the cytopathic effect for Vero cells, and the presence of pathogenic N. australiensis in the swimming pools did not correlate with bacterial indicators.
Temperature Tolerance of Pathogenic and Nonpathogenic Free-Living Amoebas
Within tested strains of the genera Naegleria and Acanthamoeba the ability to grow at high temperatures seems directly related to virulence, with nonvirulent strains unable to grow at normal or
A comparative study of 14 strains of Naegleria australiensis demonstrates the existence of a highly virulent subspecies: N. australiensis italica n. spp.
Naegleria australiensis does not appear to be as homogenous a species as N. fowleri and seems to be more widespread than previously thought.
Comparative Study of Six Strains of Naegleria with Special Reference to Nonpathogenic Variants of Naegleria fowleri
There exists an intermediate form between N. gruberi and N. fowleri, with a strong relationship to the latter species, and strains of Naegleria isolated from a body of water polluted with thermal effluents were compared.
Identification of Leishmania spp. by multiple isozyme analysis.
Calculations from the CAE data on average polymorphism indicated that Leishmania species/types or groups can be expected to be about 25% polymorphic, which suggests that isolate pairs which have profiles about 75% or more identical should be considered samples from the same species/type, and isolates that are significantly less than 75% identical are therefore samples from different species/ types.