Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme.

@article{Eugenio2007BiochemicalET,
  title={Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme.},
  author={Laura Isabel de Eugenio and Pedro de Moura Garcia and Jos{\'e} M Luengo and Jes{\'u}s M Sanz and Julio San Rom{\'a}n and J{\'e}ssica Leite Garcia and Mar{\'i}a Auxiliadora Prieto},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 7},
  pages={4951-62}
}
Polyhydroxyalkanoates (PHAs) can be catabolized by many microorganisms using intra- or extracellular PHA depolymerases. Most of our current knowledge of these intracellular enzyme-coding genes comes from the analysis of short chain length PHA depolymerases, whereas medium chain length PHA (mcl-PHA) intracellular depolymerization systems still remained to be characterized. The phaZ gene of some Pseudomonas putida strains has been identified only by mutagenesis and complementation techniques as… CONTINUE READING

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