Biochemical evidence for transcytotic absorption of polyaspartamide from the rat lung: effects of temperature and metabolic inhibitors.

@article{Sakagami2002BiochemicalEF,
  title={Biochemical evidence for transcytotic absorption of polyaspartamide from the rat lung: effects of temperature and metabolic inhibitors.},
  author={Masahiro Sakagami and Peter R. Byron and Franti{\vs}ek Ryp{\'a}{\vc}ek},
  journal={Journal of pharmaceutical sciences},
  year={2002},
  volume={91 9},
  pages={1958-68}
}
Airway-to-perfusate polyhydroxyethylaspartamide (PHEA) absorption was studied in the isolated perfused rat lung at a reduced temperature and by the use of metabolic inhibitors, to kinetically clarify the mechanisms and cellular pathways of its active absorption. Fluorophore-labeled PHEA (F-PHEA; 7.4 kDa) was administered into the airways, and its absorption followed with time at 25 degrees C and in the presence of 2,4-dinitrophenol (DNP), ouabain (OUA), monensin (MON), and nocodazole (NOC… CONTINUE READING