Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts.

@article{Luben1976BiochemicalCW,
  title={Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts.},
  author={Richard A. Luben and Glenda L. Wong and David V. Cohn},
  journal={Endocrinology},
  year={1976},
  volume={99 2},
  pages={526-34}
}
Two metabolically distinct types of bone cell populations were isolated from mouse calvaria by a repetitive digestive procedure with a mixture of collagenase and trypsin. Cells released early in the digestion showed approximately two-fold increases in cAMP when treated with either parathormone or calcitonin. These populations were denoted CT type. Later eluting cells showed larger parathormone-induced increases in cAMP but did not respond to calcitonin. These populations were denoted PT type… CONTINUE READING

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