Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro.

@article{Michel1995BiochemicalCO,
  title={Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro.},
  author={Raquel Michel and C Massanz and Susanne Kostka and Megan Richter and Klaus M. Fiebig},
  journal={European journal of biochemistry},
  year={1995},
  volume={233 3},
  pages={727-35}
}
The membrane-associated coenzyme F420-reactive hydrogenase of the anaerobic methanogenic archaeon Methanosarcina barkeri Fusaro has been purified 95-fold to apparent homogeneity. A new purification procedure and altered storage conditions gave substantially higher yield (13.4% versus 4.3%) and specific coenzyme F420-reducing activity (82.8 mumol.min-1.mg… CONTINUE READING