Biochemical characterization of the first fungal glycoside hydrolyase family 3 β-N-acetylglucosaminidase from Rhizomucor miehei.

@article{Yang2014BiochemicalCO,
  title={Biochemical characterization of the first fungal glycoside hydrolyase family 3 β-N-acetylglucosaminidase from Rhizomucor miehei.},
  author={Shaoqing Yang and Shuang Song and Qiao-juan Yan and Xing Fu and Zheng-qiang Jiang and Xinbin Yang},
  journal={Journal of agricultural and food chemistry},
  year={2014},
  volume={62 22},
  pages={5181-90}
}
A novel β-N-acetylglucosaminidase gene (RmNag) from Rhizomucor miehei was cloned and expressed in Escherichia coli. RmNag shares the highest identity of 37% with a putative β-N-acetylglucosaminidase from Aspergillus clavatus. The recombinant enzyme was purified to homogeneity. The optimal pH and temperature of RmNag were pH 6.5 and 50 °C, respectively. It was stable in the pH range 6.0-8.0 and at temperatures below 45 °C. RmNag exhibited strict substrate specificity for p-nitrophenyl β-N… CONTINUE READING