Biochemical characterization of regulatory factors derived from T cell hybridomas and spleen cells. I. Separation of T cell growth factor and T cell replacing factor from granulocyte-macrophage colony-stimulating factor.


We describe the molecular characteristics of T cell growth factor (TCGF), T cell replacing factor (TRF), and granulocyte-macrophage colony-stimulating factor (GM-CSF) produced by a T cell hybridoma after stimulation with concanavalin A (Con A). All three activities could be separated from Con A itself by ammonium sulfate precipitation. The TRF and TCGF… (More)