Biochemical characterization of purified zeta-carotene desaturase from Anabaena PCC 7120 after expression in Escherichia coli.

@article{Albrecht1996BiochemicalCO,
  title={Biochemical characterization of purified zeta-carotene desaturase from Anabaena PCC 7120 after expression in Escherichia coli.},
  author={Manuela Albrecht and Hartmut Linden and G erhard Sandmann},
  journal={European journal of biochemistry},
  year={1996},
  volume={236 1},
  pages={115-20}
}
A novel enzyme, zeta-carotene desaturase from the cyanobacterium Anabaena, which catalyzes the last two steps in a series of desaturations, was overexpressed in Escherichia coli. For the first time, this allowed the purification of this enzyme and subsequent enzyme kinetic studies. The enzyme was solubilized from the E. coli membranes by Chaps and purified to homogeneity by ammonium sulfate precipitation, ion-exchange and hydrophobic interaction chromatography. The correct translational start… CONTINUE READING