Biochemical characterization of isoprene synthase from Ipomoea batatas.

@article{Li2019BiochemicalCO,
  title={Biochemical characterization of isoprene synthase from Ipomoea batatas.},
  author={Meijie Li and Changqing Liu and Hailin Chen and Li Deng and Haibo Zhang and Rui Nian and Mo Xian},
  journal={Journal of bioscience and bioengineering},
  year={2019},
  volume={127 2},
  pages={
          138-144
        }
}
The bio-production process of isoprene, an essential chemical used in industry, is strongly limited by isoprene synthase. In our previous work, relatively high isoprene production was observed with isoprene synthase from Ipomoea batatas (IspSib). In this work the biochemical properties of IspSib were analyzed and compared with those of isoprene synthase from Populus alba (IspSpa) and other species. Firstly, IspSib and IspSpa were expressed, purified, and identified by SDS-PAGE and western blot… CONTINUE READING

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