Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways.

@article{Laupitz2004BiochemicalCO,
  title={Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways.},
  author={Ralf Laupitz and Stefan Hecht and Sabine Amslinger and Ferdinand Zepeck and Johannes Kaiser and Gerald Richter and Nicholas Schramek and Stefan Steinbacher and Robert Huber and Duilio Arigoni and Adelbert Bacher and Wolfgang Eisenreich and Felix Rohdich},
  journal={European journal of biochemistry},
  year={2004},
  volume={271 13},
  pages={2658-69}
}
An open reading frame (Acc. no. P50740) on the Bacillus subtilis chromosome extending from bp 184,997-186,043 with similarity to the idi-2 gene of Streptomyces sp. CL190 specifying type II isopentenyl diphosphate isomerase was expressed in a recombinant Escherichia coli strain. The recombinant protein with a subunit mass of 39 kDa was purified to apparent homogeneity by column chromatography. The protein was shown to catalyse the conversion of dimethylallyl diphosphate into isopentenyl… CONTINUE READING
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