Biochemical characterisation of extracellular phytase (myo-inositol hexakisphosphate phosphohydrolase) from a hyper-producing strain of Aspergillus niger van Teighem

@article{Vats2005BiochemicalCO,
  title={Biochemical characterisation of extracellular phytase (myo-inositol hexakisphosphate phosphohydrolase) from a hyper-producing strain of Aspergillus niger van Teighem},
  author={Purva Vats and Uttam Chand Banerjee},
  journal={Journal of Industrial Microbiology and Biotechnology},
  year={2005},
  volume={32},
  pages={141-147}
}
Aspergillus niger van Teighem, isolated in our laboratory from samples of rotten wood logs, produced extracellular phytase having a high specific activity of 22,592 units (mg protein)−1 . The enzyme was purified to near homogeneity using ion-exchange and gel-filtration chromatography. The molecular properties of the purified enzyme suggested the native phytase to be oligomeric, with a molecular weight of 353 kDa, the monomer being 66 kDa. The purified enzyme exhibited maximum activity at pH 2.5… CONTINUE READING
19 Citations
38 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 38 references

Isolation and identification of phytase producing bacterium, Enterobacter sp. 4 and enzymatic properties of phytase enzyme

  • SJ Yoon, YJ Choi, +4 authors YH Jung
  • Enzyme Microb Technol
  • 1996
Highly Influential
4 Excerpts

Production studies and catalytic properties of phytases (myo-inositol hexakis phosphate phos- phor hydrolases): an overview

  • P Vats, UC Banerjee
  • Enzyme Microb Technol
  • 2004
1 Excerpt

Studies on the production of phytase by a newly isolated sp. of Aspergillus niger van teigham obtained from rotten wood-logs

  • P Vats, UC Banerjee
  • Process Biochem
  • 2002
1 Excerpt

Similar Papers

Loading similar papers…