Biochemical and structural characterization of a ureidoglycine aminotransferase in the Klebsiella pneumoniae uric acid catabolic pathway.

@article{French2010BiochemicalAS,
  title={Biochemical and structural characterization of a ureidoglycine aminotransferase in the Klebsiella pneumoniae uric acid catabolic pathway.},
  author={Jarrod B. French and Steven E. Ealick},
  journal={Biochemistry},
  year={2010},
  volume={49 29},
  pages={
          5975-7
        }
}
Many plants, fungi, and bacteria catabolize allantoin as a mechanism for nitrogen assimilation. Recent reports have shown that in plants and some bacteria the product of hydrolysis of allantoin by allantoinase is the unstable intermediate ureidoglycine. While this molecule can spontaneously decay, genetic analysis of some bacterial genomes indicates that an aminotransferase may be present in the pathway. Here we present evidence that Klebsiella pneumoniae HpxJ is an aminotransferase that… 
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Given widespread concerns for crop losses due to the drastically changing climate and prevailing agricultural practices, the understanding of the interactive signaling for the purine metabolisms between PAB and plants takes on a major importance, as it may support management decisions necessary to maintain PAB biodiversity and the agricultural services provided by PAB to crops.
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Using the reaction module concept to define at least two successive steps catalyzed by homologous enzymes in pathways alignable by similar chemical reactions will help to trace how the primordial promiscuous enzymes were assembled progressively in functional modules, as the present pathways diverged from ancestral pathways to give birth to the present-day mechanistically diversified superfamilies.
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Caracterización molecular del metabolismo del alantoato en judía (Phaseolus Vulgaris)
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Los resultados obtenidos tras bloquear mediante RNAi the expresion of esta proteina sugieren that the AAH es the enzima responsable of la degradacion del alantoato en judia.
Characterization of the gene cluster involved in allantoate catabolism and its transcriptional regulation by the RpiR-type repressor HpxU in Klebsiella pneumoniae.
TLDR
Allantoate released the HpxU repressor from its target operators whereas other purine intermediate metabolites, such as allantoin and oxamate, failed to induce complex dissociation.
Biochemical and structural characterization of Klebsiella pneumoniae oxamate amidohydrolase in the uric acid degradation pathway.
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  • Biology, Chemistry
    Acta crystallographica. Section D, Structural biology
  • 2016
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The structure of HpxW is presented and the substrate complex is modeled and a mechanism for catalysis consistent with other members of the Ntn-hydrolase superfamily to be proposed is proposed.
The purinosome, a multi-protein complex involved in the de novo biosynthesis of purines in humans.
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The most recent discoveries relating to purine biosynthesis are discussed, with particular emphasis upon the dynamic multi-protein complex called the purinosome and the attempts made to exploit this knowledge for drug discovery.
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