Biochemical and structural characterization of a ureidoglycine aminotransferase in the Klebsiella pneumoniae uric acid catabolic pathway.
@article{French2010BiochemicalAS, title={Biochemical and structural characterization of a ureidoglycine aminotransferase in the Klebsiella pneumoniae uric acid catabolic pathway.}, author={Jarrod B. French and Steven E. Ealick}, journal={Biochemistry}, year={2010}, volume={49 29}, pages={ 5975-7 } }
Many plants, fungi, and bacteria catabolize allantoin as a mechanism for nitrogen assimilation. Recent reports have shown that in plants and some bacteria the product of hydrolysis of allantoin by allantoinase is the unstable intermediate ureidoglycine. While this molecule can spontaneously decay, genetic analysis of some bacterial genomes indicates that an aminotransferase may be present in the pathway. Here we present evidence that Klebsiella pneumoniae HpxJ is an aminotransferase that…
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References
SHOWING 1-10 OF 13 REFERENCES
Ureide catabolism in Arabidopsis thaliana and Escherichia coli.
- Biology, Environmental ScienceNature chemical biology
- 2010
Two enzymes are identified that catalyze the final reactions of purine degradation in the model plant Arabidopsis thaliana and an alternative metabolic route via ureidoglycine transaminase can be predicted for other organisms.
Chemical basis of nitrogen recovery through the ureide pathway: formation and hydrolysis of S-ureidoglycine in plants and bacteria.
- BiologyACS chemical biology
- 2010
NMR evidence is reported that the true product of the AAH reaction is S-ureidoglycine, a nonstandard alpha-amino acid that spontaneously releases ammonia in vitro, strengthening the basis for the known sensitivity of the ureide pathway to Mn availability and suggesting a further rationale for the active transport of Mn in the endoplasmic reticulum of plant cells.
Purine Utilization by Klebsiella oxytoca M5al: Genes for Ring-Oxidizing and -Opening Enzymes
- BiologyJournal of bacteriology
- 2009
A 23-gene cluster is identified that encodes the enzymes for utilizing purines as the sole nitrogen source in the enterobacterium Klebsiella oxytoca, and functions for the products of these genes are revealed.
Pyrimidine and purine biosynthesis and degradation in plants.
- BiologyAnnual review of plant biology
- 2006
Progress in molecular aspects and recent studies on the regulation and manipulation of nucleotide metabolism in plants are reviewed.
The hpx Genetic System for Hypoxanthine Assimilation as a Nitrogen Source in Klebsiella pneumoniae: Gene Organization and Transcriptional Regulation
- BiologyJournal of bacteriology
- 2008
The proteins involved in the oxidation of hypoxanthine to allantoin or uric acid did not display any similarity to other reported enzymes known to catalyze these reactions but instead are similar to oxygenases acting on aromatic compounds.
Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
- BiologyAnnual review of biochemistry
- 2004
The combination of new sequences and structures enables better reconstruction of their evolutionary heritage and illuminates unrecognized similarities within this diverse group of enzymes.
Genetic Analysis of a Chromosomal Region Containing Genes Required for Assimilation of Allantoin Nitrogen and Linked Glyoxylate Metabolism in Escherichia coli
- BiologyJournal of bacteriology
- 1999
A systematic analysis of the gene cluster was undertaken to identify genes involved in allantoin utilization, and the products of these genes were overexpressed and shown to have the predicted corresponding enzyme activities.
Completing the uric acid degradation pathway through phylogenetic comparison of whole genomes
- BiologyNature chemical biology
- 2006
Through phylogenetic genome comparison, this work identifies here two genes that share with urate oxidase a common history of loss or gain events and shows that the two proteins encoded by mouse genes catalyze two consecutive steps following urate oxidation to 5-hydroxyisourate (HIU): hydrolysis of HIU and decarboxylation of OHCU to give S-allantoin.
Allantoate hydrolysis by allantoate amidohydrolase.
- Biology, ChemistryArchives of biochemistry and biophysics
- 1970
Reaction of aspartate aminotransferase with C5-dicarboxylic acids: comparison with the reaction with C4-dicarboxylic acids.
- Chemistry, BiologyJournal of biochemistry
- 2003
AspAT recognizes the two types of dicarboxylates with different chain lengths by changing the gross conformation of the enzyme protein, which can be explained only when all the protonation states of the Michaelis complex are considered.