Biochemical and structural analysis of a site directed mutant of manganese dependent aminopeptidase P from Streptomyces lavendulae

@inproceedings{Nandan2015BiochemicalAS,
  title={Biochemical and structural analysis of a site directed mutant of manganese dependent aminopeptidase P from Streptomyces lavendulae},
  author={Arya S Nandan and Kesavan Madhavan Nampoothiri},
  year={2015}
}
Article info: Received: 29 August 2014 Accepted: 13 November 2014 ABSTRACT Aminopeptidase P (APP) removes N-terminal amino acids from peptides and proteins when the penultimate residue is proline. To understand the structurefunction relationships of aminopeptidase P of Streptomyces lavendulae, a conserved arginine residue was replaced with lysine (R453K) by site-directed mutagenesis. The overexpressed wild and mutant enzymes were of nearly 60 kDa and purified by nickel affinity chromatography… CONTINUE READING

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