Biochemical and physiological evidence that calmodulin is involved in the taste response of the sugar receptor cells of the blowfly, Phormia regina.
@article{Seno2005BiochemicalAP,
title={Biochemical and physiological evidence that calmodulin is involved in the taste response of the sugar receptor cells of the blowfly, Phormia regina.},
author={Keiji Seno and Tadashi Nakamura and Mamiko Ozaki},
journal={Chemical senses},
year={2005},
volume={30 6},
pages={
497-504
}
}The gustatory system is essential for almost all animals. However, the signal transduction mechanisms have not yet been fully elucidated. We isolated labellar chemosensilla from blowfly, Phormia regina, and purified calcium binding proteins from the water soluble fraction. The most abundant calcium-binding protein was calmodulin. To investigate the role of calmodulin in taste transduction, electrophysiological responses were recorded with the calmodulin inhibitor, W-7. When we stimulated the…
Figures and Tables from this paper
13 Citations
Water taste transduction pathway is calcium dependent in Drosophila.
- BiologyChemical senses
- 2009
It is shown that tarsal W cell firing activity depends on the concentration of external calcium bathing the dendrite, and suggests that the transduction pathway of hypoosmotic stimuli in insects differs from mammals.
Transduction mechanism(s) of Na-saccharin in the blowfly Protophormia terraenovae: evidence for potassium and calcium conductance involvement
- BiologyJournal of Comparative Physiology A
- 2009
The results are consistent with the hypothesis that in the labellar chemosensilla of the blowfly both Ca2+ and K+ ions are involved in the transduction mechanism of the “deterrent” cell in response to saccharin and suggest a possible pathway common to sacCharin and 8Br-cGMP.
Transduction pathways mediated by second messengers including cAMP in the sugar receptor cell of the blow fly: study by the whole cell clamp method.
- BiologyJournal of insect physiology
- 2008
Nitric oxide contributes to high-salt perception in a blood-sucking insect model
- BiologyScientific Reports
- 2017
It is demonstrated that upon high-salt detection in food a nitric oxide (NO) dependent cascade is activated, and the activation of NO/sGC/cGMP cascade commands the avoidance feeding behavior in R. prolixus.
Ca2+ stabilizes the membrane potential of moth olfactory receptor neurons at rest and is essential for their fast repolarization.
- BiologyChemical senses
- 2007
Ca(2+)-gated K(+) channels identified from cultured ORNs with whole-cell recordings are good candidates to mediate ORN repolarization, and partial inhibition of responses with a calmodulin antagonist indicates that intracellular Ca(2+) contributes to the ORN response and suggests that Ca( 2+) release from internal stores is involved.
Cyclic nucleotide-activated currents in cultured olfactory receptor neurons of the hawkmoth Manduca sexta.
- BiologyJournal of neurophysiology
- 2008
Cyclic nucleotide-activated currents in cultured olfactory receptor neurons of the moth Manduca sexta are described, which share properties with currents through vertebrate cyclicucleotide-gated channels, and suggest that the underlying channels are coupled and act coordinately.
Characterization of calcium signaling proteins from the fat body of the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae): Implications for diapause and lipid metabolism.
- BiologyInsect biochemistry and molecular biology
- 2021
Double-stranded RNA targeting calmodulin reveals a potential target for pest management of Nilaparvata lugens.
- BiologyPest management science
- 2018
BACKGROUND
Calmodulin (CaM) is an essential protein in cellular activity and plays important roles in many processes in insect development. RNA interference (RNAi) has been hypothesized to be a…
A journey into the world of insect lipid metabolism.
- BiologyArchives of insect biochemistry and physiology
- 2020
Most of the knowledge of insect lipid metabolism comes from the studies on the model Drosophila; other insects, in particular those with obligatory or facultative diapause, also have great potential to study lipid metabolism and the use of these models would significantly improve knowledge of Insect lipid metabolism.
References
SHOWING 1-10 OF 28 REFERENCES
Gqα subunit mediates receptor site-specific adaptation in the sugar taste receptor cell of the blowfly, Phormia regina
- BiologyNeuroscience Letters
- 2005
Intrinsic nitric oxide regulates the taste response of the sugar receptor cell in the blowfly, Phormia regina.
- BiologyChemical senses
- 2004
Electrophysiologically showed that NO, which may be produced by intrinsic NOS in sugar receptor cells, participates in the transduction cascade of these cells in blowfly.
Effects of cyclic GMP on the sugar taste receptor cell of the fly Phormia regina
- Biology, Chemistry
- 1990
Adaptation-promoting effect of IP3, Ca2+, and phorbol ester on the sugar taste receptor cell of the blowfly, Phormia regina
- BiologyThe Journal of general physiology
- 1992
It is hypothesized that an intrACEllular cascade involving inositol phospholipid hydrolysis, intracellular Ca2+ mobilization, and protein kinase C-mediated phosphorylation promotes adaptation of the sugar receptor cell.
Sugar reception in the blowfly: a possible Ca(++) involvement.
- BiologyJournal of insect physiology
- 2002
Inositol 1,4,5-trisphosphate transduction cascade in taste reception of the fleshfly, Boettcherisca peregrina.
- BiologyJournal of neurobiology
- 2002
The results suggest that the IP3 transduction cascade is involved in the response of the sugar receptor cell of the fly.
Perception of noxious compounds by contact chemoreceptors of the blowfly, Phormia regina: putative role of an odorant-bindingpProtein.
- BiologyChemical senses
- 2003
It is suggested that in the insect contact-chemoreceptor Sensillum, CRLBP carries lipophilic members of the noxious taste substances to the "fifth cell" through the aqueous sensillum lymph.
Nucleotide receptor-site on the labellar sugar receptor cell of the blowfly Phormia regina
- Biology, Chemistry
- 1992
Transduction Ion Channels Directly Gated by Sugars on the Insect Taste Cell
- BiologyThe Journal of general physiology
- 2000
The results strongly suggested that the channel is an ionotropic receptor (a receptor/channel complex), activated directly by sucrose without mediation by second messengers or G protein, and was shown to be a nonselective cation channel.
Two types of sugar-binding protein in the labellum of the fly. Putative taste receptor molecules for sweetness
- BiologyThe Journal of general physiology
- 1993
These proteins showed consistent sugar-binding specificities and affinities with the two types of receptor sites for sweetness, respectively, and are likely to act as the taste receptor molecules for sweetness in the fly.









