Biochemical and pharmacological characterization of the cyclooxygenase activity of human blood prostaglandin endoperoxide synthases.

@article{Patrignani1994BiochemicalAP,
  title={Biochemical and pharmacological characterization of the cyclooxygenase activity of human blood prostaglandin endoperoxide synthases.},
  author={Paola Patrignani and Maria Rosaria Panara and Anita Greco and Octavio Fusco and Clara Natoli and Stefano Iacobelli and Francesco Cipollone and Amy Ganci and Christophe A Cr{\'e}minon and Jacques Maclouf},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1994},
  volume={271 3},
  pages={1705-12}
}
The aim of our study was to characterize a model of human prostaglandin endoperoxide synthase-2 (PGHS-2) expression allowing the assessment of pharmacological inhibition in vitro and ex vivo. Heparinized human whole blood samples were incubated with lipopolysaccharide (LPS, 0.1-50 micrograms/ml) for 0 to 24 hr at 37 degrees C. The contribution of platelet PGHS-1 was suppressed by either pretreating the subjects with aspirin (300 mg 48 hr before sampling) or adding aspirin (10 micrograms/ml) in… CONTINUE READING
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