Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea.

@article{Chen2004BiochemicalAM,
  title={Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea.},
  author={Shicheng Chen and Wei Ge and John A. Buswell},
  journal={European journal of biochemistry},
  year={2004},
  volume={271 2},
  pages={318-28}
}
We have isolated a laccase (lac1) from culture fluid of Volvariella volvacea, grown in a defined medium containing 150 micro m CuSO4, by ion-exchange and gel filtration chromatography. Lac1 has a molecular mass of 58 kDa as determined by SDS/PAGE and an isoelectric point of 3.7. Degenerate primers based on the N-terminal sequence of purified lac1 and a conserved copper-binding domain were used to generate cDNA fragments encoding a portion of the lac1 protein and RACE was used to obtain full… CONTINUE READING

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