Biochemical and immunological characterization of the flagellar-associated regulatory subunit of a type II cyclic adenosine 5'-monophosphate-dependent protein kinase.

@article{Horowitz1989BiochemicalAI,
  title={Biochemical and immunological characterization of the flagellar-associated regulatory subunit of a type II cyclic adenosine 5'-monophosphate-dependent protein kinase.},
  author={J A Horowitz and Pamela J. Voulalas and Wilma Wasco and J Macleod and Marie C. Paupard and George A. Orr},
  journal={Archives of biochemistry and biophysics},
  year={1989},
  volume={270 2},
  pages={
          411-8
        }
}
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10 Citations

10 Citations

Citation Type

Citation Type

Association of the regulatory subunit of a type II cAMP-dependent protein kinase and its binding proteins with the fibrous sheath of rat sperm flagellum.
TLDR
It is shown that all three polypeptides capable of binding RII on a solid-phase blot are tightly associated with the fibrous sheath, suggesting a developmental role for RII anchoring to the sperm flagellar fibrousSheath.
Subcellular localization of the regulatory subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase in bovine spermatozoa.
TLDR
Monospecific, affinity-purified, antipeptide antibodies were used to determine the distribution of the regulatory (R) subunit isoforms and data suggest distinct roles for each of these isoforms in sperm functions such as motility and the acrosome reaction.
Cloning and characterization of a testis-specific, developmentally regulated A-kinase-anchoring protein (TAKAP-80) present on the fibrous sheath of rat sperm.
TLDR
The cloning of a rat testis-specific, developmentally regulated, RII-binding protein (TAKAP-80) is reported, which established that a type II cAMP-dependent protein kinase is tightly associated with the fibrous sheath of rat sperm.
Differential localization of two isoforms of the regulatory subunit RIIα of cAMP‐dependent protein kinase in human sperm: Biochemical and cytochemical study
In the present study, immunogold labeling of ultrathin sections of ejaculated sperm was used to obtain insight into the ultrastructural localization and presumable function of type II cAMP‐dependent…
Developmental regulation of unique adenosine 3',5'-monophosphate-specific phosphodiesterase variants during rat spermatogenesis.
TLDR
The expression of novel cAMP-specific PDEs coded by the PDE4A gene is demonstrated, which is maximal in round spermatids and is maintained in mature spermatozoa and could not be detected in Sertoli or interstitial cells.
Cyclic AMP-dependent phosphorylation of epididymal mouse sperm proteins during capacitation in vitro: identification of an M(r) 95,000 phosphotyrosine-containing protein.
TLDR
Results suggest that the protein detected may be the same phosphotyrosine-containing protein as that described by Leyton and Saling (1989) which has been proposed to play a role in acrosomal exocytosis.
Cellular location and age-dependent changes of the regulatory subunits of cAMP-dependent protein kinase in rat testis.
TLDR
In some types of cell, discrepancies between mRNA and protein were demonstrated, which clearly suggest cell specific differences in translational efficiencies for some of these PKA subunits, particularly the small-sized mRNAs for RI alpha and RII alpha in meiotic and post-meiotic germ cells.
Localization of a novel human A-kinase-anchoring protein, hAKAP220, during spermatogenesis.
Using a combination of protein kinase A type II overlay screening, rapid amplification of cDNA ends, and database searches, a contig of 9923 bp was assembled and characterized in which the open…
Impact of Epididymal Maturation on the Tyrosine Phosphorylation Patterns Exhibited by Rat Spermatozoa1
TLDR
The findings of this study help to clarify the biochemical changes associated with the functional maturation of spermatozoa during epididymal transit.
7 – Molecular Basis of Signaling in Spermatozoa

References

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Immunogold localization of the regulatory subunit of a type II cAMP- dependent protein kinase tightly associated with mammalian sperm flagella
TLDR
The results raise the possibility that a type II cAMP-dependent protein kinase located at the outer mitochondrial membrane plays a role in the direct cAMP stimulation of mitochondrial respiration during sperm activation.
Interaction of the regulatory subunit of a type II cAMP-dependent protein kinase with mammalian sperm flagellum.
TLDR
Flagellar polypeptides which are capable of forming tight complexes with the regulatory subunit of type II cAMP-dependent protein kinase (RII) are identified and it is proposed that RII is released from the flagellum, a highly disulfide cross-linked structure, due to structural changes in the flagesllum which disrupts the interaction between RII and its binding proteins.
Isolation and sequence of a tryptic peptide containing the autophosphorylation site of the regulatory subunit of bovine brain protein kinase II.
The regulatory subunit monomer of cAMP-dependent protein kinase retains the salient kinetic properties of the native dimeric subunit.
The regulatory subunit of neural cAMP-dependent protein kinase II represents a unique gene product.
Microheterogeneity of type II cAMP-dependent protein kinase in various mammalian species and tissues.
Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes
Localization of the regulatory subunit of type II cyclic AMP-dependent protein kinase on the cytoplasmic microtubule network of cultured cells.
Major 56,000‐dalton, soluble phosphoprotein present in bovine sperm is the regulatory subunit of a type II cAMP‐dependent protein kinase
TLDR
The data suggest that the major 56,000 dalton cAMP‐dependent phosphoprotein present in bovine sperm is the regulatory subunit of a type II cAMP•dependent protein kinase and not the motility initiator protein, axokinin.
The neural type II regulatory subunit of cAMP-dependent protein kinase is present and regulated by hormones in the rat ovary.
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