Biochemical and immunochemical studies of proteolytic fragments of exotoxin A from Pseudomonas aeruginosa.

@article{Bourdenet1990BiochemicalAI,
  title={Biochemical and immunochemical studies of proteolytic fragments of exotoxin A from Pseudomonas aeruginosa.},
  author={Sylviane Bourdenet and M J. Vacheron and M Guinand and Georges Michel and F. Arminjon},
  journal={European journal of biochemistry},
  year={1990},
  volume={192 2},
  pages={379-85}
}
Limited proteolysis of Pseudomonas aeruginosa exotoxin A by four proteases (chymotrypsin, Staphylococcal serine proteinase, pepsin A and subtilisin) resulted in the formation of polypeptides having a molecular mass of approximately 25 kDa. They possessed both enzymatic activity and residual antigenicity. Their N-terminal sequence analysis showed that the… CONTINUE READING