Biochemical and genetic characterization of a gentisate 1, 2-dioxygenase from Sphingomonas sp. strain RW5.

Abstract

A 4,103-bp long DNA fragment containing the structural gene of a gentisate 1,2-dioxygenase (EC 1.13.11.4), gtdA, from Sphingomonas sp. strain RW5 was cloned and sequenced. The gtdA gene encodes a 350-amino-acid polypeptide with a predicted size of 38.85 kDa. Comparison of the gtdA gene product with protein sequences in databases, including those of… (More)

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@article{Werwath1998BiochemicalAG, title={Biochemical and genetic characterization of a gentisate 1, 2-dioxygenase from Sphingomonas sp. strain RW5.}, author={J Werwath and H A Arfmann and Dietmar H. Pieper and Kenneth N. Timmis and R M Wittich}, journal={Journal of bacteriology}, year={1998}, volume={180 16}, pages={4171-6} }