Biochemical and genetic characterization of a competence pheromone from B. subtilis

@article{Magnuson1994BiochemicalAG,
  title={Biochemical and genetic characterization of a competence pheromone from B. subtilis},
  author={Roy David Magnuson and Jonathan M. Solomon and Alan D. Grossman},
  journal={Cell},
  year={1994},
  volume={77},
  pages={207-216}
}
Characterization of a competence pheromone in Bacillus subtilis
TLDR
These two signaling pathways converge to induce expression of srfA, a locus that is required for the production of surfactin, the development of competence and for efficient sporulation, which may be important for the coordination of social strategies.
Cell-cell signaling and the regulation of development in Bacillus subtilis
TLDR
Synthetic CSF has the ability to stimulate sporulation at low cell density, indicating that CSF is a sporulation factor as well as a competence factor.
Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis.
TLDR
Characterization of a second extracellular competence stimulating factor (CSF) appears to be, at least in part, a small peptide of between 520 and 720 daltons, which might function to integrate different physiological signals.
Identification of the streptococcal competence‐pheromone receptor
TLDR
Evidence is obtained demonstrating that the histidine kinase ComD is the competence‐pheromone receptor of Streptococcus pneumoniae strain Rx, and tracing the CSP specificity of the competence response in these strains to strain‐specific alleles of comD, it is speculated that their products, a histidine Kinase (ComD and its cognate response regulator (ComE), act downstream of the C SP in competence regulation.
Isolation and characterization of mutations that alter the quorum response in Bacillus subtilis
TLDR
By using CSF to integrate information on cell density and physiology cells can change the cell density at which the quorum response is activated in response to nutritional conditions, and this thesis demonstrates the connection between carbon and energy physiology and cell-cell signaling.
Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto‐induced peptide pheromone and a two‐component regulatory system
The regulation of competence for genetic transformation in Streptococcus pneumoniae depends on a quorum‐sensing system, but the only molecular elements of the system whose specific role have been
Competence for transformation: a matter of taste.
Purification and characterization of an extracellular peptide factor that affects two different developmental pathways in Bacillus subtilis.
TLDR
It is proposed that CSF is transported into the cell by the Spo0K oligopeptide permease and stimulates competence gene expression by inhibiting (either directly or indirectly) the RapC phosphatase.
Specificity and Genetic Polymorphism of theBacillus Competence Quorum-Sensing System
TLDR
It is shown that coexpression of comQ and comX in Escherichia coli leads to the production of active pheromone in the medium, demonstrating that comQ is the only dedicated protein required for the processing, modification, and release of active competence phermone.
...
...

References

SHOWING 1-10 OF 68 REFERENCES
Genetic competence in Bacillus subtilis.
TLDR
This review summarizes the present state of knowledge concerning competence in B. subtilis and proposes a provisional scheme for the organization of this pathway.
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis
TLDR
A role for ComQ early in the hierarchy of competence regulatory genes, probably as a component of a signal transduction system is suggested.
A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems.
TLDR
DNA sequencing of the new determinant, named comP, revealed that the carboxy-terminal domain of the predicted ComP protein is similar in amino acid sequence to that of several sensor members of the bacterial two-component signal-transduction systems.
Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants
TLDR
The complete nucleotide sequences of the comA and comB loci of Bacillus subtilis were determined and the predicted protein sequence of the COMA ORF1 gene product was found to be similar to that of several members of the effector class of procaryotic signal transducers.
Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis
TLDR
The sequence of the complementing DNA revealed that the srfB gene is comA, an early competence gene which codes for a product similar to that of the activator class of bacterial two-component regulatory systems, suggesting that surfactin production is positively regulated at the transcriptional level by the srfA (comA) gene product.
Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon
TLDR
Complementation analysis confirmed that the comG ORF1 protein is required for the ability of competent cultures to resolve into two populations with different cell densities on Renografin (E. R. Squibb & Sons, Princeton, N.J.) gradients, as well as for full expression of comE, another late competence locus.
Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function
TLDR
The data indicated that pCF10 endows its host E. faecalis cell with the ability to specifically remove cCF10 activity from culture medium by irreversible binding, and DNA sequencing revealed three open reading frames which have been denoted prgW (pheromone responsive gene W), prgZ, and prgY.
The oligopeptide transport system of Bacillus subtilis plays a role in the initiation of sporulation
TLDR
It is postulate that the accumulation of such peptides may play a signaling role in the initiation of sporulation, and that the sporulation defect in opp mutants results from an inability to transport these peptides.
Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor.
TLDR
This work has isolated deletion mutations that remove part of the conserved amino terminus of Spo0A and make the transcription factor constitutively active, indicating that the aminoterminus normally functions to keep the protein in an inactive state.
ComA, a phosphorylated response regulator protein of Bacillus subtilis, binds to the promoter region of srfA
TLDR
It is shown that partially purified ComA protein, isolated from an overproducing Escherichia coli strain, is phosphorylated in vitro by incubation with acetyl phosphate and that ComA could bind specifically to a DNA fragment containing the promoter of srfA and associated sequences.
...
...