Biochemical and genetic analysis of toxic effect of HOE 15030 in mammalian cells.

@article{Ishida1989BiochemicalAG,
  title={Biochemical and genetic analysis of toxic effect of HOE 15030 in mammalian cells.},
  author={Ryoji Ishida and Makoto Nishizawa and F Kohtani and Toshitada Takahashi},
  journal={Somatic cell and molecular genetics},
  year={1989},
  volume={15 4},
  pages={279-88}
}
HOE 15030 inhibited the growth of BHK cells at concentrations that did not inhibit their nuclear DNA and RNA syntheses. When BHK cells were cultured in the presence of 30 micrograms/ml of HOE 15030, cells were arrested in the G1 phase after one or two cell divisions. After removal of the drug, cells progressed through the G1 to the S phase. HOE 15030 inhibited the activities of both topoisomerases I and II in vitro. To determine the target molecule of HOE 15030 in cells, we isolated a HOE 15030… CONTINUE READING