Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.


Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control. Analysis of 5573 MORFs demonstrates that nearly all verified ORFs are expressed, suggests the authenticity of 48 ORFs characterized as dubious, and implicates specific processes including cytoskeletal organization and transcriptional control in growth inhibition caused by overexpression. Global analysis of glycosylated proteins identifies 109 new confirmed N-linked and 345 candidate glycoproteins, nearly doubling the known yeast glycome.

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@article{Gelperin2005BiochemicalAG, title={Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.}, author={Daniel M Gelperin and Michael A. White and Martha L Wilkinson and Yoshiko Kon and Li A. Kung and Kevin J. Wise and Nelson Lopez-Hoyo and Lixia Jiang and Stacy Piccirillo and Haiyuan Yu and Mark B Gerstein and Mark E Dumont and Eric M. Phizicky and Michael Snyder and Elizabeth J. Grayhack}, journal={Genes & development}, year={2005}, volume={19 23}, pages={2816-26} }