Biochemical and cellular investigation of Vitreoscilla hemoglobin (VHb) variants possessing efficient peroxidase activity.

@article{IsarankuraNaAyudhya2010BiochemicalAC,
  title={Biochemical and cellular investigation of Vitreoscilla hemoglobin (VHb) variants possessing efficient peroxidase activity.},
  author={Chartchalerm Isarankura-Na-Ayudhya and Natta Tansila and Apilak Worachartcheewan and Leif B{\"u}low and Virapong Prachayasittikul},
  journal={Journal of microbiology and biotechnology},
  year={2010},
  volume={20 3},
  pages={532-41}
}
Peroxidase-like activity of Vitreoscilla hemoglobin (VHb) has been recently disclosed. To maximize such activity, two catalytically conserved residues (histidine and arginine) found in distal pocket of peroxidases have successfully been introduced into that of the VHb. Fifteen-fold increase in catalytic constant (k(cat)) was obtained in P54R variant, which… CONTINUE READING