Biochemical and Structural Studies of Uncharacterized Protein PA0743 from Pseudomonas aeruginosa Revealed NAD+-dependent l-Serine Dehydrogenase*

@article{Tchigvintsev2011BiochemicalAS,
  title={Biochemical and Structural Studies of Uncharacterized Protein PA0743 from Pseudomonas aeruginosa Revealed NAD+-dependent l-Serine Dehydrogenase*},
  author={A. Tchigvintsev and A. Singer and G. Brown and Robert L Flick and E. Evdokimova and K. Tan and C. Gonzalez and A. Savchenko and A. Yakunin},
  journal={The Journal of Biological Chemistry},
  year={2011},
  volume={287},
  pages={1874 - 1883}
}
  • A. Tchigvintsev, A. Singer, +6 authors A. Yakunin
  • Published 2011
  • Biology, Medicine
  • The Journal of Biological Chemistry
    • Background: β-Hydroxyacid dehydrogenases are ubiquitous enzymes, most of which remain uncharacterized. Results: Biochemical, crystallographic, and mutational analyses identified uncharacterized Pseudomonas aeruginosa protein PA0743 as a l-serine dehydrogenase and characterized the molecular details of its active site. Conclusion: PA0743 is the first NAD+-dependent l-serine dehydrogenase potentially involved in serine catabolism. Significance: Our study provides molecular insights into the… CONTINUE READING
    View on ASBMB
    jbc.org
    16 Citations
    Highly Influential Citations
    2
    Background Citations
    5
    Methods Citations
    1
    16 Citations
    Citation Type

    Citation Type

    Substrate Diversity of L-Threonic Acid Dehydrogenase Homologs
    Characterization of a novel class of glyoxylate reductase belonging to the β-hydroxyacid dehydrogenase family in Acetobacter aceti
    Structural and biochemical characterization of the Bacillus cereus 3-hydroxyisobutyrate dehydrogenase.
    • 7
    Structural characterization of a β-hydroxyacid dehydrogenase from Geobacter sulfurreducens and Geobacter metallireducens with succinic semialdehyde reductase activity.
    • 12
    Structure, interactions and action of Mycobacterium tuberculosis 3-hydroxyisobutyric acid dehydrogenase.
    • 1
    Cloning, Expression and Characterization of 3-Hydroxyisobutyrate Dehydrogenase from Pseudomonas denitrificans ATCC 13867
    • 11
    • PDF
    Crystal structure of the NADP+ and tartrate-bound complex of l-serine 3-dehydrogenase from the hyperthermophilic archaeon Pyrobaculum calidifontis
    3-hydroxyisobutyrate dehydrogenase-I from Pseudomonas denitrificans ATCC 13867 degrades 3-hydroxypropionic acid
    • 12
    Identification of 4-Deoxy-L-Etychro-Hexoseulose Uronic Acid Reductases in an Alginolytic Bacterium Vibrio splendidus and their Uses for L-Lactate Production in an Escherichia coli Cell-Free System
    • 1
    Crystallization and preliminary X-ray analysis of L-serine 3-dehydrogenase complexed with NADP+ from the hyperthermophilic archaeon Pyrobaculum calidifontis.
    • 1
    • Highly Influenced
    • PDF

    References

    SHOWING 1-10 OF 67 REFERENCES
    Novel β-Hydroxyacid Dehydrogenases in Escherichia coli and Haemophilus influenzae *
    • 20
    • PDF
    Crystal structure of novel NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8.
    • 31
    New developments in our understanding of the -hydroxyacid dehydrogenases
    • 23
    New developments in our understanding of the beta-hydroxyacid dehydrogenases.
    • 9
    Crystal structure of serine dehydrogenase from Escherichia coli: important role of the C-terminal region for closed-complex formation.
    • 17
    • PDF
    Structural and kinetic properties of a beta-hydroxyacid dehydrogenase involved in nicotinate fermentation.
    • 12
    An inducible NADP(+)-dependent D-phenylserine dehydrogenase from Pseudomonas syringae NK-15: purification and biochemical characterization.
    • 10
    Conformational changes associated with cofactor/substrate binding of 6-phosphogluconate dehydrogenase from Escherichia coli and Klebsiella pneumoniae: Implications for enzyme mechanism.
    • 22
    • PDF
    Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C).
    • 27
    Structural Insight into Substrate Differentiation of the Sugar-metabolizing Enzyme Galactitol Dehydrogenase from Rhodobacter sphaeroides D*
    • 18
    • PDF