Biochemical analysis of hypermutational targeting by wild type and mutant activation-induced cytidine deaminase.

@article{Bransteitter2004BiochemicalAO,
  title={Biochemical analysis of hypermutational targeting by wild type and mutant activation-induced cytidine deaminase.},
  author={Ronda Bransteitter and Phuong Quoc Pham and Peter P Calabrese and Myron F. Goodman},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 49},
  pages={51612-21}
}
The synthesis of high affinity antibodies requires activation-induced cytidine deaminase (AID) to initiate somatic hypermutation and class-switch recombination. Here we investigate AID-catalyzed deamination of C --> U on single-stranded DNA and on actively transcribed closed circular double-stranded DNA. Mutations are initially favored at canonical WRC (W = A or T, R = A or G) somatic hypermutation hot spot motifs, but over time mutations at neighboring non-hot spot sites increase creating… CONTINUE READING

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