Biochemical Characterization of the Human Cyclin-dependent Protein Kinase Activating Kinase

  title={Biochemical Characterization of the Human Cyclin-dependent Protein Kinase Activating Kinase},
  author={Ann Yee and Lingtao Wu and Ling Liu and Ryuji Kobayashi and Yue Xiong and Frederick L. Hall},
  journal={The Journal of Biological Chemistry},
  pages={471 - 477}
The activation of cyclin-dependent protein kinases (Cdks) is dependent upon site-specific phosphorylation and dephosphorylation reactions, as well as positive and negative regulatory subunits. The human Cdk-activating protein kinase (Cak1) is itself a Cdc2-related cyclin-dependent protein kinase that associates with cyclin H. The present study utilized specific anti-Cak1 antibodies and immunoaffinity chromatography to identify additional Cak1-associated proteins and potential target substrates… 

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