Biochemical Characterization of Haloalkane Dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily

@article{Jesenska2009BiochemicalCO,
  title={Biochemical Characterization of Haloalkane Dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily},
  author={Andrea Jesenská and Marta Monincov{\'a} and Tana Koudelakova and Khomaini Hasan and Radka Chaloupkova and Zbynek Prokop and Arie Geerlof and Jiř{\'i} Damborsk{\'y}},
  journal={Applied and Environmental Microbiology},
  year={2009},
  volume={75},
  pages={5157 - 5160}
}
ABSTRACT This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases. 
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